Complexation between Amylodextrin Oligomers and Selected Pharmaceuticals Measured through Capillary Electrophoresis

Area of Science:

• Analytical Chemistry
• Separation Science
• Pharmaceutical Analysis

Background:

• Carbohydrate-drug interactions are crucial in pharmaceutical development.
• Capillary electrophoresis (CE) offers high-resolution separation capabilities.
• Amylodextrins are versatile cyclodextrins with applications in chiral recognition.

Purpose of the Study:

• To investigate carbohydrate-drug interactions using fluorescently tagged amylodextrin oligomers as model solutes.
• To evaluate the impact of pharmaceutical additives on amylodextrin separation patterns.
• To achieve enantiomeric resolution of pharmaceuticals utilizing amylodextrins as chiral selectors in CE.

Main Methods:

• Capillary electrophoresis with laser fluorescence detection.
• Utilizing fluorescently tagged amylodextrin oligomers.
• Employing pharmaceuticals as buffer additives to study complexation.
• Investigating the influence of solution chemistry (pH, ionic strength, additives) on interactions.
• Complementary analysis using (13)C NMR spectroscopy.

Main Results:

• Significant alterations in amylodextrin oligomer separation patterns were observed in the presence of pharmaceutical additives.
• Complexation selectivity was discernible from changes in migration times and peak shapes.
• Complex formation was sensitive to the chemical environment and the nature of guest molecules.
• Successful enantiomeric resolution of several pharmaceuticals was achieved using amylodextrins as chiral selectors.

Conclusions:

• Capillary electrophoresis is effective for studying carbohydrate-drug interactions and characterizing complexation.
• Amylodextrins serve as valuable chiral selectors for the enantiomeric separation of pharmaceuticals.
• Solution conditions and molecular structure significantly influence carbohydrate-drug complexation.