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Updated: Jun 1, 2026

Real-Time Metabolic Detection in Living Cells Using Hyperpolarized 13C NMR
Published on: July 8, 2025
(13)C dynamic nuclear polarization: an alternative detector for recycled-flow NMR experiments
S Stevenson1, T Glass, H C Dorn
1Department of Chemistry, Virginia Polytechnic Institute and State University, Blacksburg, Virginia 24061.
Abstract:
Static NMR experiments on insensitive nuclei (e.g., (13)C) have been generally difficult because of low magnetogyric ratios and/or poor natural abundance, even at the highest magnetic fields available. One method of overcoming these sensitivity limitations is dynamic nuclear polarization (DNP), which can be utilized to enhance NMR signals relative to those obtained with static NMR detection. In this study, we report a recycled-flow DNP apparatus capable of monitoring (13)C scalar-dominated enhancements 1-2 orders of magnitude greater than those obtainable using the conventional recycled-flow (13)C NMR experiment. Specifically, a mixture of benzene and several chlorocarbons were continuously "recycled" through a (13)C DNP spectrometer. Results indicate successful detection with the (13)C DNP approach in scalar-dominated cases, where analytes could not be observed with conventional static or flow NMR detection in a reasonable length of time.
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