Related Experiment Video
Updated: Jun 1, 2026
Generation and Functional Verification of Hypoxia-Sensitive Chimeric Antigen Receptor-T Cells
Published on: June 14, 2024
Novel IFNγ ELISPOT assay for detection of functional carcinoembryonic antigen-specific chimeric antigen
1Centre for Biochemistry, Drug Design and Cancer Research, School of Environment & Life Sciences, University of Salford, Salford, UK. e.elkord@salford.ac.uk
Abstract:
We here describe the development of a novel ELISPOT assay for the detection and enumeration of IFNγ-secreting functional chimeric antigen receptor (CAR)-redirected T cells against carcinoembryonic antigen (CEA). This method is valuable for clinical trials to monitor the presence of functional CEA-specific T cells transduced with a CAR. The same principle should be applicable for the detection of functional CAR-redirected T cells against any other tumour-associated antigens by immobilizing a particular biotinylated antigen to streptavidin-coated beads.
