Vestibulo-ocular projections in the 11-day chicken embryo: pathway specificity

The organization of vestibulo-ocular projections and their spatial relationship to vestibulo-spinal projections were ascertained in the 11-day chicken embryo through retrograde tracing experiments. An in vitro preparation of the brainstem facilitated precisely localized application of tracers. Unilateral labelling of the ascending medial longitudinal fascicle (MLF), just caudal to the trochlear nucleus, labelled coherent groups of vestibular neurons that had an asymmetrical distribution on the two sides of the brainstem. Axons originating from either side followed one of four trajectories, and the groups of neurons could therefore be identified on the basis of their position and their projection pathway. Labelling each side of the MLF with a different tracer showed that although neurons projecting in the ipsi- and contralateral MLF were intermingled in some areas, individual neurons projected on one side only. Labelling the ascending MLF and the high cervical spinal cord with different tracers showed that vestibulo-ocular neurons had a wider rostro-caudal distribution than vestibulospinal neurons. Some vestibulospinal and vestibulo-ocular groups were spatially segregated, others were intermingled. Very few (in most preparations no) neurons were found to project to both targets. Together with our previous study on vestibulospinal projections in the 11-day chicken embryo (Glover and Pétursdóttir, J. Comp. Neurol. 270:25-38, '88) the results show that vestibular neurons in different regions project in characteristic subsets of the available pathways.