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Induction of Invasive Transitional Cell Bladder Carcinoma in Immune Intact Human MUC1 Transgenic Mice: A Model for Immunotherapy Development
Published on: October 30, 2013
Proteomics research on muscle-invasive bladder transitional cell carcinoma
Hai Tao Niu1, Zhen Dong1, Gang Jiang2
1Department of Urology, The Affiliated Hospital of Medical College Qingdao University, Qingdao, China.
Cancer Cell International
|June 8, 2011
Summary
This study compared proteomes of muscle-invasive bladder cancer and normal cells, identifying proteins for biomarker selection and multi-target therapy. Findings offer insights into cancer
Area of Science:
- Proteomics
- Cancer Biology
- Biomarker Discovery
Background:
- Comparative proteomics research on muscle-invasive bladder transitional cell carcinoma.
- Utilized laser capture microdissection for purified cell harvesting.
- Employed two-dimensional liquid chromatography tandem mass spectrometry for proteome profiling.
Purpose of the Study:
- Facilitate candidate biomarker selection.
- Improve network-based multi-target therapy strategies.
- Identify differential protein expression in bladder cancer.
Main Methods:
- Laser capture microdissection of tumor and normal urothelial cells.
- Two-dimensional liquid chromatography tandem mass spectrometry (LC-MS/MS).
- Bioinformatic analysis and comparison with published literature.
Main Results:
- Identified 885/890 commonly expressed proteins across 4 paired samples.
- Annotated 295/337 tumor/normal specific proteins with Gene Ontology (GO) cellular components.
- Revealed significant pathway changes including spliceosome and endocytosis.
Conclusions:
- Established the proteome expression profile for muscle-invasive bladder cancer cells.
- Provided information for subcellular pattern research in cancer.
- Offered candidate proteins for biomarker panels and network-based therapies.
