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Related Experiment Video

Updated: Jun 1, 2026

Implementation of In Vitro Drug Resistance Assays: Maximizing the Potential for Uncovering Clinically Relevant Resistance Mechanisms

Implementation of In Vitro Drug Resistance Assays: Maximizing the Potential for Uncovering Clinically Relevant Resistance Mechanisms

Published on: December 9, 2015

Genotypic resistance testing in routine clinical care.

David T Dunn¹, Kate Coughlin, Patricia A Cane

¹MRC Clinical Trials Unit, London, UK. d.dunn@ctu.mrc.ac.uk

Current Opinion in HIV and AIDS

|June 8, 2011

Summary

Genotypic resistance testing for HIV is rapidly advancing, with new tools aiding treatment optimization. While interpretation challenges persist, emerging methods detect resistance at lower viral loads and identify minority variants.

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Last Updated: Jun 1, 2026

Implementation of In Vitro Drug Resistance Assays: Maximizing the Potential for Uncovering Clinically Relevant Resistance Mechanisms

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Published on: December 9, 2015

An Affordable HIV-1 Drug Resistance Monitoring Method for Resource Limited Settings

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Prediction of HIV-1 Coreceptor Usage (Tropism) by Sequence Analysis using a Genotypic Approach

Prediction of HIV-1 Coreceptor Usage (Tropism) by Sequence Analysis using a Genotypic Approach

Published on: December 1, 2011

Area of Science:

Virology
Clinical Medicine
Genetics

Background:

Genotypic resistance testing is integral to managing HIV-infected patients.
Recent advancements focus on improving the clinical utility of these tests.

Purpose of the Study:

To review recent advances in genotypic resistance testing for HIV.
To highlight challenges and developments in interpreting resistance test results.

Main Methods:

Focus on observational studies.
Review of recent technological and interpretative developments in genotypic resistance testing.

Main Results:

Therapy optimization tools now predict effective regimens for virological suppression.
Expanded testing targets beyond protease and reverse transcriptase are needed.
Mutations can be detected at viral loads below 1000 copies/ml.
Methods for detecting minority variants, including allele-specific PCR and deep sequencing, have been developed.

Conclusions:

Genotypic resistance testing technology and interpretation are rapidly evolving.
The integration of new developments into routine clinical practice remains uncertain.