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Related Experiment Video

Updated: Jun 1, 2026

Analysis of Gene Expression Changes in the Rat Hippocampus After Deep Brain Stimulation of the Anterior Thalamic Nucleus

Analysis of Gene Expression Changes in the Rat Hippocampus After Deep Brain Stimulation of the Anterior Thalamic Nucleus

Published on: March 8, 2015

Activity-dependent neuroprotective protein modulates its own gene expression.

Moutasem S Aboonq¹, Sylvia A Vasiliou, Kate Haddley

¹Institute of Translational Medicine, Sherrington Building, Ashton Street, University of Liverpool, Liverpool, L69 3GE, UK.

Journal of Molecular Neuroscience : MN

|June 8, 2011

Summary

Activity-dependent neuroprotective protein (ADNP) can regulate its own gene expression. Studies show ADNP binds to its promoter, acting as a repressor to control its own levels.

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Published on: August 28, 2021

Area of Science:

Neuroscience
Molecular Biology
Gene Regulation

Background:

Activity-dependent neuroprotective protein (ADNP) plays a crucial role in neuroprotection.
The mechanisms by which ADNP expression is controlled are not fully understood.

Purpose of the Study:

To investigate the potential for activity-dependent neuroprotective protein (ADNP) to autoregulate its own gene expression.
To determine if ADNP acts as a repressor of its own promoter.

Main Methods:

Overexpression of wild-type and mutant ADNP (lacking the NAP motif).
Analysis of endogenous ADNP gene expression.
Reporter gene assays to assess ADNP promoter activity.
Chromatin immunoprecipitation (ChIP) to detect ADNP binding to its promoter.

Main Results:

Overexpression of both wild-type and mutant ADNP led to decreased endogenous ADNP expression.
ADNP overexpression repressed reporter gene activity driven by the ADNP promoter.
Chromatin immunoprecipitation confirmed that ADNP binds to its own promoter.

Conclusions:

Activity-dependent neuroprotective protein (ADNP) autoregulates its expression by acting as a repressor.
ADNP binds to its own promoter, inhibiting both endogenous gene expression and promoter-driven transcription.