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Automated micropipette tip-based SPE in quantitative bioanalysis.

Jacquelynn Luckwell1, Andrew Beal

  • 1Bioanalytical Group, Pharmacokinetics, Dynamics & Metabolism, Pfizer Global Research & Development, Sandwich, Kent, CT13 9NJ, UK. jacqui.luckwell@pfizer.com

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|June 9, 2011
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Summary
This summary is machine-generated.

Automated micropipette tip solid-phase extraction (SPE) significantly reduces sample preparation time for small molecule analysis. This cost-effective method accelerates preclinical and clinical studies by optimizing liquid chromatography-tandem mass spectrometry workflows.

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Area of Science:

  • Analytical Chemistry
  • Biotechnology
  • Pharmacology

Background:

  • Development of automated methodologies for small molecule analysis.
  • Application in preclinical and first-in-human studies using LC-MS/MS.
  • Utilizes silica-based, monolithic, micropipette tip-based solid-phase extraction (SPE).

Purpose of the Study:

  • To outline the use of micropipette tip-based SPE with automated liquid handling systems.
  • To evaluate prototype micropipette tips for robotic platforms.
  • To develop efficient sample preparation methods for small molecule analysis.

Main Methods:

  • Implementation of mixed-mode cation exchange and C18 SPE methods.
  • Utilized human and rat plasma matrices.
  • Employed automated liquid handling systems (Tomtec Quadra 96 and Hamilton Microlab Star).

Main Results:

  • Successful extraction of lipophilic and polar analytes.
  • Achieved low plasma sample, washing, and elution volumes.
  • Demonstrated a rapid method extraction cycle time of approximately 6.2 minutes for 96 samples.

Conclusions:

  • Automated SPE significantly reduces extraction time compared to 96-well plate formats.
  • Sample preparation is no longer the rate-limiting step in selective extraction.
  • Developed methods are robust, sensitive, and more cost-effective than traditional SPE.