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Related Experiment Videos

Chromate reduction in Streptomyces.

S Das1, A L Chandra

  • 1Department of Microbiology, Bose Institute, Calcutta, India.

Experientia
|July 15, 1990
PubMed
Summary
This summary is machine-generated.

Streptomyces species 3M detoxify hexavalent chromium (Cr6+) using a constitutive enzyme. This enzyme, found in a particulate cell fraction, reduces Cr6+ to Cr3+ with NADH.

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Area of Science:

  • Microbiology
  • Environmental Science
  • Biochemistry

Background:

  • Hexavalent chromium (Cr6+) is a toxic heavy metal pollutant.
  • Microbial remediation offers a sustainable approach to heavy metal detoxification.
  • Streptomyces species are known for their metabolic diversity and potential in bioremediation.

Purpose of the Study:

  • To investigate the ability of Streptomyces species 3M to reduce hexavalent chromium.
  • To identify the cellular components involved in chromate reduction.
  • To characterize the enzyme responsible for Cr6+ detoxification.

Main Methods:

  • Cultivation of Streptomyces species 3M in the presence of potassium dichromate (K2Cr2O7).
  • Fractionation of cell components via high-speed centrifugation (105,000 x g).

Related Experiment Videos

  • Assay of Cr6+ reduction in the presence of NADH and NADPH cofactors.

    • Main Results:

    • Streptomyces species 3M demonstrated growth in the presence of 1000 µg/ml K2Cr2O7.
    • A particulate cell fraction significantly reduced Cr6+ levels by 82.7% with NADH.
    • The Cr6+-reducing activity was attributed to a constitutive enzyme associated with the particulate fraction, converting Cr6+ to Cr3+.

    Conclusions:

    • Streptomyces species 3M possesses a robust system for detoxifying hexavalent chromium.
    • A specific particulate enzyme, likely a reductase, plays a key role in this detoxification pathway.
    • This finding supports the potential of Streptomyces for bioremediation of chromium-contaminated environments.