Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

MALDI-TOF Mass Spectrometry01:19

MALDI-TOF Mass Spectrometry

Mass spectrometry is a powerful characterization technique that can identify and separate a wide variety of compounds ranging from chemical to biological entities, based on their mass-to-charge ratio (m/z). The instruments that allow this detection, known as mass spectrometers, have three components: an ion source, a mass analyzer, and a detector. These spectrometers differ based on the nature of their ion source and analyzers.Matrix-assisted laser desorption ionization (MALDI) is a commonly...
Rapid Identification of Pathogens01:25

Rapid Identification of Pathogens

MALDI-TOF MS has transformed clinical microbiology by offering a rapid and reliable method for pathogen identification. The traditional approach to microbial identification typically involves time-consuming culture techniques and biochemical tests, which can delay the initiation of appropriate antimicrobial therapy. MALDI-TOF MS avoids these delays by using characteristic ribosomal protein mass patterns of microbial cells, enabling accurate species-level identification within minutes.Principle...
Peptide Identification Using Tandem Mass Spectrometry01:33

Peptide Identification Using Tandem Mass Spectrometry

Tandem mass spectrometry, also known as MS/MS or MS2, is an analytical technique that employs two mass analyzers. Essentially it is a series of mass spectrometers that helps isolate a particular biomolecule and then helps study its chemical properties.
This technique helps gather information regarding the protein from which the peptide was obtained and to study the peptides’ amino acid sequence. Identifying peptides from a complex mixture is an important component of the growing field of...
Matrix-Assisted Laser Desorption Ionization (MALDI)01:08

Matrix-Assisted Laser Desorption Ionization (MALDI)

Matrix-assisted laser desorption ionization (MALDI) is a powerful analytical technique used in mass spectrometry. It enables the identification and characterization of various biomolecules, including proteins, peptides, nucleic acids, and carbohydrates. MALDI is an ionization technique, widely employed in biological and medical research, as well as in fields like pharmacology and biochemistry.The analyte of interest, a biomolecule or a mixture of biomolecules, is mixed with a suitable matrix...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Assessing Intraoperative Tumor-to-Background Ratios Across Different Subsites of the Oral Cavity Using an Integrin-Specific Fluorescent Tracer.

Cancers·2026
Same author

Flavonoid-rich dietary patterns and the risk of incident hearing loss: evidence from the UK Biobank cohort.

The journal of nutrition, health & aging·2026
Same author

Real-Time Detection of Colorectal Cancer Using Topical Application of a pH-Activatable Fluorophore.

Molecular diagnosis & therapy·2026
Same author

Near-infrared fluorescence imaging-guided targeted drug delivery in gastric tumors: From molecular design to clinical translation.

Advanced drug delivery reviews·2026
Same author

Zwitterionic [Gd-(DOTA)] MRI Probes: Influence of Sulfobetaine Linker Length on Relaxivity.

ACS omega·2026
Same author

Zwitterionic Mn(III)-Tetraphenyl Porphyrins: Water-Soluble MRI Contrast Agents with High Relaxivity.

ChemMedChem·2026

Related Experiment Video

Updated: Jun 1, 2026

Low Molecular Weight Protein Enrichment on Mesoporous Silica Thin Films for Biomarker Discovery
13:00

Low Molecular Weight Protein Enrichment on Mesoporous Silica Thin Films for Biomarker Discovery

Published on: April 17, 2012

High-throughput small molecule identification using MALDI-TOF and a nanolayered substrate.

Jeong Heon Lee1, Hak Soo Choi, Khaled A Nasr

  • 1Robotic Chemistry Group, Center for Molecular Imaging, Beth Israel Deaconess Medical Center, Boston, Massachusetts 02215, USA.

Analytical Chemistry
|June 10, 2011
PubMed
Summary

This study introduces a novel nanolayered substrate and system for high-throughput small molecule identification in encoderless combinatorial chemistry, achieving 100% accuracy. This advancement enhances chemical synthesis and drug discovery processes.

More Related Videos

MALDI Sample Preparation: the Ultra Thin Layer Method
05:28

MALDI Sample Preparation: the Ultra Thin Layer Method

Published on: April 29, 2007

Matrix-assisted Laser Desorption/Ionization Time of Flight (MALDI-TOF) Mass Spectrometric Analysis of Intact Proteins Larger than 100 kDa
07:49

Matrix-assisted Laser Desorption/Ionization Time of Flight (MALDI-TOF) Mass Spectrometric Analysis of Intact Proteins Larger than 100 kDa

Published on: September 9, 2013

Related Experiment Videos

Last Updated: Jun 1, 2026

Low Molecular Weight Protein Enrichment on Mesoporous Silica Thin Films for Biomarker Discovery
13:00

Low Molecular Weight Protein Enrichment on Mesoporous Silica Thin Films for Biomarker Discovery

Published on: April 17, 2012

MALDI Sample Preparation: the Ultra Thin Layer Method
05:28

MALDI Sample Preparation: the Ultra Thin Layer Method

Published on: April 29, 2007

Matrix-assisted Laser Desorption/Ionization Time of Flight (MALDI-TOF) Mass Spectrometric Analysis of Intact Proteins Larger than 100 kDa
07:49

Matrix-assisted Laser Desorption/Ionization Time of Flight (MALDI-TOF) Mass Spectrometric Analysis of Intact Proteins Larger than 100 kDa

Published on: September 9, 2013

Area of Science:

  • Chemistry
  • Analytical Chemistry
  • Chemical Engineering

Background:

  • Encoderless combinatorial chemistry enables rapid synthesis and identification of novel molecules without tags.
  • High-throughput screening is crucial for accelerating drug discovery and materials science.
  • Existing methods for small molecule identification face limitations in speed and accuracy.

Purpose of the Study:

  • To develop and validate a high-throughput system for encoderless small molecule identification.
  • To optimize a novel nanolayered substrate and integrated analytical workflow.
  • To establish a foundation for advanced combinatorial chemistry applications.

Main Methods:

  • Development of a nanolayered substrate plate.
  • Integration with a microarraying robot for precise sample handling.
  • Application of matrix-assisted laser desorption/ionization (MALDI) mass spectrometry.
  • Utilization of custom-developed software, including a proofreading and recalibration algorithm.

Main Results:

  • Achieved 100% spot and molecule identification accuracy for over 100,000 molecules.
  • Demonstrated high mass accuracy as low as 45 ppm through an internal recalibration algorithm.
  • Successfully identified 47 diverse chemical entities with 100% accuracy in a separate validation set.

Conclusions:

  • The developed system provides a robust and highly accurate solution for high-throughput small molecule identification.
  • This technology significantly advances the capabilities of encoderless combinatorial chemistry.
  • The findings pave the way for more efficient synthesis and discovery of novel chemical compounds.