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Preparing Adherent Cells for X-ray Fluorescence Imaging by Chemical Fixation
Published on: March 12, 2015
An Application of X-ray Fluorescence Spectrometry to the Study of Thin-Layer Chromatography
1National Food Research Institute, Ministry of Agriculture, Forestry and Fisheries, Tsukuba, Ibaraki 305, Japan.
Abstract:
The combination of thin-layer chromatography (TLC) with X-ray fluorescence spectrometry (XRF) has been accomplished without any interfaces. It enables the direct, nondestructive visualization of elements developed on a TLC plate. In addition to inorganic compounds, organic compounds including electronegative elements can be detected simultaneously that cannot always be measured satisfactorily by competitive techniques; e.g., phenolic compounds containing chlorine, bromine, or iodine were distinctly detected by individual elemental imaging. The background of commercially available TLC plates with various thicknesses of the stationary phase and an outline of element detection limits were also investigated. TLC/XRF was found suitable for in situ TLC imaging of elements.
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