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Updated: Jun 1, 2026

A Protocol for Detecting and Scavenging Gas-phase Free Radicals in Mainstream Cigarette Smoke
Published on: January 2, 2012
Detection and separation of gas-phase carbon-centered radicals from cigarette smoke and diesel exhaust
1Chemistry Department, Michigan Technological University, Houghton, Michigan 49931-1295.
Abstract:
Carbon-centered radicals were trapped from gas-phase cigarette smoke and diesel engine exhaust by reaction with a nitroxide, 3-amino-2,2,5,5-tetramethyl-1-pyrrolidinyloxy (3AP). The resulting mixture of stable, diamagnetic adducts was derivatized with naphthalenedicarboxaldehyde (NDA) to produce highly fluorescent products. Derivatives were separated by high-performance liquid chromatography (HPLC), which revealed distinctly different suites of radicals present in the two systems. Integration of HPLC peaks gave approximately 22 ± 7 nmol of radicals per cigarette and 3 ± 1 nmol of radicals per liter of diesel engine exhaust. An estimated 8-10 different carbon-centered radical species are present in each system.
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