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[Effect of UBE2C overexpression on the proliferation of 293T cell line]
Xiang Huang1, Yu-Lin Qiu, Jia-Yu Tan
1Department of Lab Medicine, Boai Hospital Affiliated With Southern Medical University, Zhongshan 528403, China.
Aim:
To establish a 293T cell line with stable expression of UBE2C and to investigate the effect of UBE2C overexpression on the proliferation of 293T cells.
Methods:
Recombinant plasmid pcDNA3.1(-)/UBE2C successfully constructed in previous time was transfected into 293T cells by lipofectamine 2000. After screened with G418 for 4 weeks, Western blot was used to detect the protein level of UBE2C and MTT assay to detect the proliferation of 293T cells.
Results:
Compared with pcDNA3.1(-) transfected group, Western blot analysis demonstrated that the level of UBE2C was significantly increased in pcDNA3.1(-)/UBE2C transfected group (P < 0.05). MTT assay showed that 293T cells overexpressing UBE2C proliferated faster than control 293T cells.
Conclusion:
A 293T cell line with UBE2C stable expression is successfully established and UBE2C promoted the proliferation of 293T cells.
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