Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Ubiquitin immunoreactive structures in normal human brains. Distribution and developmental aspects.

D W Dickson1, A Wertkin, Y Kress

  • 1Department of Pathology (Neuropathology), Albert Einstein College of Medicine, Bronx, New York.

Laboratory Investigation; a Journal of Technical Methods and Pathology
|July 1, 1990
PubMed
Summary

Ubiquitin immunostaining reveals age-related changes in normal human brains, identifying dystrophic neurites and axonal spheroids as common findings that increase with age.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Association of amyloid angiopathy with microbleeds in logopenic progressive aphasia: an imaging-pathology study.

European journal of neurology·2020
Same author

Reproduction of <i>Meloidogyne enterolobii</i> on selected root-knot nematode resistant sweetpotato (<i>Ipomoea batatas</i>) cultivars.

Journal of nematology·2020
Same author

A First Report of Paulownia elongata as a Host of Meloidogyne spp. in Florida.

Plant disease·2019
Same author

First Report of Meloidogyne mayaguensis on Angelonia angustifolia.

Plant disease·2019
Same author

First Report of Meloidogyne enterolobii Infecting Euphorbia punicea in Florida.

Plant disease·2019
Same author

First Report of Laurel Oak as a Host for the Pecan Root-Knot Nematode, Meloidogyne partityla, in Florida.

Plant disease·2019

Area of Science:

  • Neuroscience
  • Cell Biology
  • Pathology

Background:

  • Ubiquitin is a protein involved in protein degradation and cellular stress responses.
  • Age-related changes in the brain are complex and not fully understood.
  • Identifying normal age-related alterations is crucial for distinguishing them from disease pathology.

Purpose of the Study:

  • To characterize ubiquitin-immunoreactive structures in normal human brains across a wide age range.
  • To determine the prevalence and distribution of these structures in both gray and white matter.
  • To investigate the cellular and subcellular localization of ubiquitin-immunoreactive components.

Main Methods:

  • Light and electron microscopy were used to examine brain tissue from individuals aged 2 months to 91 years.

Related Experiment Videos

  • Antibodies against ubiquitin were employed for immunostaining.
  • Immunoelectron microscopy provided detailed ultrastructural analysis.

    • Main Results:

    • Ubiquitin immunostaining highlighted dystrophic neurites in cortical layers and amygdala nuclei, increasing with age.
    • White matter showed more numerous, smaller "dot-like" ubiquitin-immunoreactive structures compared to gray matter.
    • Axonal spheroids were found in specific brain regions from the second decade onwards, increasing with age.

    Conclusions:

    • Progressive axonal dystrophy and glial alterations are common age-related changes in the normal human brain.
    • Ubiquitin immunostaining is a valuable tool for visualizing these age-dependent structural modifications.
    • These findings suggest that axonal dystrophy may be a more prevalent age-related brain alteration than previously recognized.