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Related Experiment Video

Updated: Jun 1, 2026

Characterizing Salmonella Typhimurium-induced Septic Peritonitis in Mice
14:10

Characterizing Salmonella Typhimurium-induced Septic Peritonitis in Mice

Published on: July 29, 2022

Systemic mast cell degranulation increases mortality during polymicrobial septic peritonitis in mice.

Eric J Seeley1, Rachel E Sutherland, Sophia S Kim

  • 1Department of Medicine, University of California, San Francisco, CA, USA.

Journal of Leukocyte Biology
|June 10, 2011
PubMed
Summary

Systemic mast cells (MCs) worsen survival during sepsis, unlike local MCs that aid bacterial clearance. Targeting systemic MC activation may improve outcomes in septic peritonitis.

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Area of Science:

  • Immunology
  • Pathophysiology
  • Host-Pathogen Interactions

Background:

  • Mast cells (MCs) are crucial for effective host defense in septic peritonitis.
  • Local MC degranulation aids neutrophil recruitment, activation, and bacterial clearance.
  • The role of distant MCs during sepsis remains unclear.

Purpose of the Study:

  • To investigate the temporal and spatial degranulation of MCs in a mouse model of septic peritonitis.
  • To evaluate the functional importance of systemic MC degranulation during infection using compartment-specific MC reconstitution.

Main Methods:

  • Cecal ligation and puncture (CLP) model of septic peritonitis in mice.
  • Measurement of serum histamine as a marker of MC degranulation.
  • Histological examination of MC degranulation in various tissues.

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  • Compartment-specific reconstitution of MC-deficient mice.

    • Main Results:

    • Serum histamine levels increased 4 hours post-CLP, indicating MC degranulation.
    • Progressive MC degranulation was observed in specific tissues within 24 hours post-CLP.
    • MC-deficient mice reconstituted only in the peritoneal cavity showed improved survival.
    • Reconstitution in both peritoneal and systemic compartments worsened survival.
    • IL-6 mediated the detrimental effects of systemic MCs, as IL-6(-/-) MC reconstitution improved survival.

    Conclusions:

    • Local MC activation benefits host response during sepsis.
    • Systemic MC activation exacerbates mortality in CLP-induced sepsis.
    • Interleukin-6 plays a key role in the negative impact of systemic MCs on survival.