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Exon Recombination

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The evolution of new genes is critical for speciation. Exon recombination, also known as exon shuffling or domain shuffling, is an important means of new gene formation. It is observed across vertebrates, invertebrates, and in some plants such as potatoes and sunflowers. During exon recombination, exons from the same or different genes recombine and produce new exon-intron combinations, which might evolve into new genes.
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Related Experiment Video

Updated: Jun 1, 2026

Preparation of the Mgm101 Recombination Protein by MBP-based Tagging Strategy

Preparation of the Mgm101 Recombination Protein by MBP-based Tagging Strategy

Published on: June 25, 2013

Cdc45: the missing RecJ ortholog in eukaryotes?

Luis Sanchez-Pulido¹, Chris P Ponting

¹Department of Physiology, Anatomy and Genetics, MRC Functional Genomics Unit, University of Oxford, Oxford OX1 3QX, UK. luis.sanchezpulido@dpag.ox.ac.uk

Bioinformatics (Oxford, England)

|June 10, 2011

Summary

This summary is machine-generated.

Researchers traced the evolutionary history of Cdc45, an essential protein in eukaryotic DNA replication. Computational analysis revealed a common ancestry with prokaryotic RecJ, suggesting Cdc45 has an exonuclease domain and catalytic site.

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Area of Science:

Molecular Biology
Evolutionary Biology
Genetics

Background:

DNA replication is a fundamental cellular process conserved across all life.
Cdc45 is a crucial protein in eukaryotic DNA replication, essential for initiation and elongation.
The precise molecular function of Cdc45 remains largely unknown.

Purpose of the Study:

To investigate the evolutionary history of the Cdc45 protein family.
To identify potential functional domains within Cdc45.
To elucidate the molecular function of Cdc45 through comparative analysis.

Main Methods:

Computational sequence analysis of the Cdc45 protein family.
Comparative genomics to identify homologous proteins.
Bioinformatic prediction of protein domains and catalytic sites.

Main Results:

Eukaryotic Cdc45 and prokaryotic RecJ proteins share a common evolutionary origin.
Cdc45 possesses a predicted exonuclease domain.
A catalytic site is identified within the predicted exonuclease domain of Cdc45.

Conclusions:

The findings suggest a conserved ancestral function between Cdc45 and RecJ.
Cdc45 likely functions as an enzyme with exonuclease activity in DNA replication.
This discovery opens new avenues for understanding eukaryotic DNA replication mechanisms.