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Updated: Jun 1, 2026

Identification of Rare Bacterial Pathogens by 16S rRNA Gene Sequencing and MALDI-TOF MS
Published on: July 11, 2016
Rapid identification of Cryptococcus neoformans and Cryptococcus gattii by matrix-assisted laser desorption
Lisa R McTaggart1, Eric Lei, Susan E Richardson
1Mycology Department, Ontario Agency for Health Protection and Promotion, 81 Resources Rd., Toronto, Ontario M9P 3T1, Canada. lisa.mctaggart@oahpp.ca
Abstract:
Compared to DNA sequence analysis, matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) correctly identified 100% of Cryptococcus species, distinguishing the notable pathogens Cryptococcus neoformans and C. gattii. Identification was greatly enhanced by supplementing a commercial spectral library with additional entries to account for subspecies variability.
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