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Updated: Jun 1, 2026

qPCR Is a Sensitive and Rapid Method for Detection of Cytomegaloviral DNA in Formalin-fixed, Paraffin-embedded Biopsy Tissue
Published on: July 9, 2014
Diagnostic consequences of cytomegalovirus glycoprotein B polymorphisms
Zdenek Novak1, Nazma Chowdhury, Shannon A Ross
1University of Alabama at Birmingham, Children's Hospital, CHB 150, 1600 6th Ave. S., Birmingham, AL 35233, USA. zdnovak@peds.uab.edu
None:
Failure of a cytomegalovirus (CMV) real-time PCR assay targeting glycoprotein B (gB) was investigated. A multiplex assay targeting gB and immediate-early 2 (IE2) genes showed discordant results (gB negative and IE positive or a >10-fold-higher viral load with IE primers) in saliva from 14.6% of CMV-infected newborns. Sequencing revealed 3 patterns of gB variations.
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