Integrated and diffusion-based micro-injectors for open access cell assays

Area of Science:

• Biotechnology
• Cell Biology
• Microfluidics

Background:

• Traditional microfluidic devices are complex and not user-friendly for routine laboratory cell processing.
• Current in vitro cell processing relies on conventional labware like petri dishes and multi-well plates.
• There is a need for accessible microfluidic tools for biological research.

Purpose of the Study:

• To develop an open-access microfluidic device for simplified in vitro cell processing.
• To enable localized drug delivery and precise control of molecular gradients.
• To demonstrate the utility of the device in cell-based assays.

Main Methods:

• Fabrication of an open-access device with an array of isolated micro-channels on a culture surface.
• Incorporation of micro-nozzles for localized molecule delivery.
• Control of molecular concentration gradients by adjusting flow rates within micro-channels.
• Demonstration using cell staining and drug-induced apoptosis assays.

Main Results:

• The open-access device facilitates routine operation in standard biology laboratories.
• Steady molecular concentration gradients were achieved in a diffusion-dominant regime.
• The device enabled rapid cell responses to localized drug delivery.
• Successful demonstration of cell staining and drug-induced apoptosis.

Conclusions:

• The developed open-access microfluidic device offers a user-friendly alternative for in vitro cell processing.
• Precise control over molecular gradients can be achieved for various cell-based assays.
• This technology has the potential to advance cell biology research and drug screening.