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Related Experiment Video

Updated: Jun 1, 2026

Single-cell Microinjection for Cell Communication Analysis
09:59

Single-cell Microinjection for Cell Communication Analysis

Published on: February 26, 2017

Integrated and diffusion-based micro-injectors for open access cell assays.

Xin Li1, Li Liu, Li Wang

  • 1Ecole Normale Supérieure, CNRS-ENS-UPMC UMR 8640, 24 rue Lhomond, 75005, Paris, France.

Lab on a Chip
|June 10, 2011
PubMed
Summary
This summary is machine-generated.

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This study introduces an open-access microfluidic device with isolated micro-channels for localized drug delivery. This innovation simplifies in vitro cell processing in biology labs, enabling precise gradient control for cell assays.

Area of Science:

  • Biotechnology
  • Cell Biology
  • Microfluidics

Background:

  • Traditional microfluidic devices are complex and not user-friendly for routine laboratory cell processing.
  • Current in vitro cell processing relies on conventional labware like petri dishes and multi-well plates.
  • There is a need for accessible microfluidic tools for biological research.

Purpose of the Study:

  • To develop an open-access microfluidic device for simplified in vitro cell processing.
  • To enable localized drug delivery and precise control of molecular gradients.
  • To demonstrate the utility of the device in cell-based assays.

Main Methods:

  • Fabrication of an open-access device with an array of isolated micro-channels on a culture surface.
  • Incorporation of micro-nozzles for localized molecule delivery.

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Last Updated: Jun 1, 2026

Single-cell Microinjection for Cell Communication Analysis
09:59

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Published on: February 26, 2017

A Microfluidic Chip for the Versatile Chemical Analysis of Single Cells
15:41

A Microfluidic Chip for the Versatile Chemical Analysis of Single Cells

Published on: October 15, 2013

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12:25

Counting Proteins in Single Cells with Addressable Droplet Microarrays

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  • Control of molecular concentration gradients by adjusting flow rates within micro-channels.
  • Demonstration using cell staining and drug-induced apoptosis assays.

    • Main Results:

    • The open-access device facilitates routine operation in standard biology laboratories.
    • Steady molecular concentration gradients were achieved in a diffusion-dominant regime.
    • The device enabled rapid cell responses to localized drug delivery.
    • Successful demonstration of cell staining and drug-induced apoptosis.

    Conclusions:

    • The developed open-access microfluidic device offers a user-friendly alternative for in vitro cell processing.
    • Precise control over molecular gradients can be achieved for various cell-based assays.
    • This technology has the potential to advance cell biology research and drug screening.