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Related Experiment Video

Updated: Jun 1, 2026

Generation and Purification of Human INO80 Chromatin Remodeling Complexes and Subcomplexes

Generation and Purification of Human INO80 Chromatin Remodeling Complexes and Subcomplexes

Published on: October 23, 2014

SWI/SNF chromatin remodeling complex: a new cofactor in reprogramming.

Ling He¹, Huan Liu, Liling Tang

¹Key Laboratory of Biorheological Science and Technology, Ministry of Education, College of Bioengineering, Chongqing University, Chongqing, 400044, China.

Stem Cell Reviews and Reports

|June 10, 2011

Summary

The SWItch/Sucrose NonFermentable (SWI/SNF) chromatin remodeling complex is crucial for reprogramming somatic cells into induced pluripotent stem (iPS) cells. It enhances reprogramming factor binding, increasing efficiency for potential new therapies.

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Generation and Purification of Human INO80 Chromatin Remodeling Complexes and Subcomplexes

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Biochemical Assays for Analyzing Activities of ATP-dependent Chromatin Remodeling Enzymes

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Published on: October 25, 2014

Area of Science:

Stem Cell Biology
Epigenetics
Chromatin Remodeling

Background:

Induced pluripotent stem (iPS) cells are generated from somatic cells using four key factors: Oct4, Sox2, Klf4, and c-Myc.
The SWItch/Sucrose NonFermentable (SWI/SNF) chromatin remodeling complex has emerged as a critical player in cellular reprogramming and stem cell pluripotency maintenance.

Purpose of the Study:

To review recent advances in understanding the role of the SWI/SNF complex in somatic cell reprogramming.
To discuss the potential mechanisms by which SWI/SNF influences reprogramming efficiency and pluripotency.

Main Methods:

Literature review of recent studies on SWI/SNF complex function in cellular reprogramming.
Analysis of proposed mechanisms involving SWI/SNF's interaction with reprogramming factors and gene promoters.

Main Results:

SWI/SNF complex enhances the binding of reprogramming factors (Oct4, Sox2, Klf4, c-Myc) to pluripotent gene promoters.
This enhanced binding activity is proposed to increase the overall efficiency of somatic cell reprogramming into iPS cells.

Conclusions:

The SWI/SNF complex plays a vital role in facilitating somatic cell reprogramming by modulating chromatin accessibility.
Understanding SWI/SNF's mechanism offers insights into reprogramming processes and potential therapeutic applications, including patient-specific stem cell generation.