Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Mesenchymal Stem Cells

Mesenchymal Stem Cells

Mesenchymal stem cells (MSCs) are adult stem cells that can differentiate into most connective tissue cell types, except for hematopoietic cells, depending upon the source of MSCs. For example, bone-marrow-derived MSCs (BM-MSCs) can differentiate into osteocytes, hepatocytes, and pancreatic and neuronal cells. MSCs can be isolated from various sources such as bone marrow, placenta, adipose tissue, teeth, and Wharton’s jelly, a gelatinous substance in the umbilical cord. The ease of their access...

Basal Lamina are the Specialized Form of ECM

Basal Lamina are the Specialized Form of ECM

The basal lamina is a thin extracellular layer that lies underneath the cells and separates them from other tissues. The three layers of the basal lamina are lamina lucida, lamina densa and lamina reticularis. The basal lamina, a mixture of glycoproteins and collagen, provides an attachment site for the epithelium, separating it from underlying connective tissue. The framework of basal lamina has other essential proteins such as laminins mesh, perlecan, entactin, and type IV collagen.
Proteins...

The Extracellular Matrix

The Extracellular Matrix

Overview
In order to maintain tissue organization, many animal cells are surrounded by structural molecules that make up the extracellular matrix (ECM). Together, the molecules in the ECM maintain the structural integrity of tissue as well as the remarkable specific properties of certain tissues.
Composition of the Extracellular Matrix
The extracellular matrix (ECM) is commonly composed of ground substance, a gel-like fluid, fibrous components, and many structurally and functionally diverse...

Overview of Cell-Matrix Interactions

Overview of Cell-Matrix Interactions

The extracellular matrix or ECM holds cells together to form a tissue and allows the cells within the tissue to communicate. ECM comprises proteins such as fibronectin, collagen, laminin, etc. The most abundant protein in this space is collagen. Collagen fibers are interwoven with carbohydrate-containing protein molecules called proteoglycans. ECM allows cell migration and provides a structural scaffold at cell adhesion that anchors the cell when the extracellular matrix proteins interact with...

Extracellular Matrix

Extracellular Matrix

Unlike epithelial tissue, which is composed of cells closely packed with little or no extracellular space in between, connective tissue cells are dispersed in a matrix. This extracellular matrix (ECM) is composed of fibrous proteins like collagen, elastin, and fibronectin in a ground substance consisting of interstitial fluid, cell adhesion proteins, and proteoglycans. The proteoglycans form a gel-like material in the spaces between cells and provide hydration, buffering, binding, and force...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Novel murine model provides insights into early-onset of kidney disease in glycogen storage disease type Ib.

Molecular genetics and metabolism·2025

Same author

The Wnt/β-catenin signaling pathway mediates renal fibrosis in glycogen storage disease type Ib nephropathy.

Biochimica et biophysica acta. Molecular basis of disease·2025

Same author

Base-editing corrects metabolic abnormalities in a humanized mouse model for glycogen storage disease type-Ia.

Nature communications·2024

Same author

Inhibition of Wnt/β-catenin signaling reduces renal fibrosis in murine glycogen storage disease type Ia.

Biochimica et biophysica acta. Molecular basis of disease·2023

Same author

CRISPR/Cas9-based double-strand oligonucleotide insertion strategy corrects metabolic abnormalities in murine glycogen storage disease type-Ia.

Journal of inherited metabolic disease·2023

Same author

0.1% RGN-259 (Thymosin ß4) Ophthalmic Solution Promotes Healing and Improves Comfort in Neurotrophic Keratopathy Patients in a Randomized, Placebo-Controlled, Double-Masked Phase III Clinical Trial.

International journal of molecular sciences·2023

Search research articles

Related Experiment Video

Updated: Jun 1, 2026

Comparative Study of Basement-Membrane Matrices for Human Stem Cell Maintenance and Intestinal Organoid Generation

Comparative Study of Basement-Membrane Matrices for Human Stem Cell Maintenance and Intestinal Organoid Generation

Published on: March 15, 2024

Basement membrane matrix (BME) has multiple uses with stem cells.

Irina Arnaoutova¹, Jay George, Hynda K Kleinman

¹Trevigen Inc., 8405 Helgerman Ct., Gaithersburg, MD 29877, USA.

Stem Cell Reviews and Reports

|June 10, 2011

Summary

This summary is machine-generated.

Basement membrane extract (BME) effectively addresses challenges in stem cell research, improving survival, proliferation, and differentiation. This review details BME methods for enhanced stem cell applications in vitro and in vivo.

More Related Videos

Ultrathin Porated Elastic Hydrogels As a Biomimetic Basement Membrane for Dual Cell Culture

Ultrathin Porated Elastic Hydrogels As a Biomimetic Basement Membrane for Dual Cell Culture

Published on: December 26, 2017

Basement Membrane Matrix Encapsulated Cell Aggregation for Investigating Murine Spleen Tissue Formation

Basement Membrane Matrix Encapsulated Cell Aggregation for Investigating Murine Spleen Tissue Formation

Published on: June 28, 2024

Related Experiment Videos

Last Updated: Jun 1, 2026

Comparative Study of Basement-Membrane Matrices for Human Stem Cell Maintenance and Intestinal Organoid Generation

Comparative Study of Basement-Membrane Matrices for Human Stem Cell Maintenance and Intestinal Organoid Generation

Published on: March 15, 2024

Ultrathin Porated Elastic Hydrogels As a Biomimetic Basement Membrane for Dual Cell Culture

Ultrathin Porated Elastic Hydrogels As a Biomimetic Basement Membrane for Dual Cell Culture

Published on: December 26, 2017

Basement Membrane Matrix Encapsulated Cell Aggregation for Investigating Murine Spleen Tissue Formation

Basement Membrane Matrix Encapsulated Cell Aggregation for Investigating Murine Spleen Tissue Formation

Published on: June 28, 2024

Area of Science:

Stem Cell Biology
Biomaterials Science
Tissue Engineering

Background:

Stem cell research faces hurdles like difficult extraction, feeder layer dependency, and poor survival/proliferation/differentiation.
Basement membrane, the embryo's first extracellular matrix, is crucial for modulating stem cell behavior.
Basement membrane extract (BME) has emerged as a key biomaterial to overcome these limitations.

Purpose of the Study:

To review current methodologies utilizing basement membrane extract (BME) in stem cell research.
To highlight BME's role in overcoming obstacles in stem cell extraction, culture, and transplantation.
To demonstrate BME's utility as both an in vitro substratum and an in vivo bioactive support.

Main Methods:

Utilizing thin BME coatings to maintain undifferentiated embryonic stem cell phenotypes during expansion.
Employing thick BME hydrogels to induce stem cell differentiation.
Leveraging BME as a bioactive support for in vivo stem cell survival and co-culture applications.

Main Results:

BME successfully maintains stem cell pluripotency and promotes proliferation in vitro.
BME facilitates controlled stem cell differentiation when applied as a hydrogel.
BME significantly enhances stem cell survival and integration in vivo transplantation models.

Conclusions:

Basement membrane extract (BME) is a versatile biomaterial that significantly advances stem cell research.
BME provides a physiological niche that supports stem cell maintenance, expansion, differentiation, and in vivo efficacy.
Current BME methodologies offer solutions for previously intractable problems in stem cell biology and therapeutic applications.