Related Experiment Video
Updated: Jun 1, 2026

Sexual Development and Ascospore Discharge in Fusarium graminearum
Published on: March 29, 2012
Characterization of a hydrophobin of the ascomycete Paecilomyces farinosus
Stefan Lunkenbein1, Meike Takenberg, Manfred Nimtz
1Zentrum Angewandte Chemie der Leibniz Universität Hannover, Institut für Lebensmittelchemie, Callinstrasse 5, Hannover, Germany. stefan.lunkenbein@lci.uni-hannover.de
Abstract:
The entomopathogenic ascomycete Paecilomyces farinosus (alternative name Isaria farinosa) synthesized a hydrophobin, irrespective of being grown in submerged or surface culture. The protein was extracted using trifluoroacetic acid and purified using preparative HPLC and SDS-PAGE. Partial sequences were obtained using ESI-MS/MS. The peptides were used as a start to apply a 'template switching oligo' protocol to elucidate the complete open reading frame of P. farinosus hydrophobin 1 (pfah1). The deduced protein sequence comprised 107 amino acids (10.7 kDa) including a 16 amino acid long hydrophobic signal peptide, showed a calculated pI of 4.56, and was interrupted by one intron. Phylogenetic analyses revealed relationships to hydrophobins of the ascomycetes Magnaporthe grisea and Metarhizium anisopliae. Based on solubility, hydropathy pattern and phylogeny PfaH1 was assigned to the class Ia hydrophobins.
Related Concept Videos
Fungal Phylum Ascomycota
Fungal Group Zygomycota
Bacterial Phylum Actinobacteria
Antifungal Agents

