Decay of nitroprusside. I: In vitro

The apparent intravascular decomposition of nitroprusside (SNP) has been attributed to photolysis and artefactual generation of cyanide (HCN) during assay, leading some workers to believe that large doses of SNP may be infused safely if light is excluded. However, we have shown that HCN is not produced from SNP during analysis. Significant amounts of HCN were formed only when SNP was first incubated with blood. The yield of HCN was a function of the temperature, pH and time of incubation. The time for release of 50% of the HCN from SNP 5 mumol litre-1 at 37 degrees C in blood was 26.6 min with greater than 90% yield in 2 h, and in plasma the optimum pH was about 7.5. A u.v. method for measuring SNP suggests that, at clinically appropriate blood concentrations, SNP is confined to plasma.