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Updated: Jun 1, 2026
Rapid Molecular Detection and Differentiation of Influenza Viruses A and B
Published on: January 30, 2017
Longitudinal molecular microbial analysis of influenza-like illness in New York City, May 2009 through May 2010
Rafal Tokarz1, Vishal Kapoor, Winfred Wu
1Center for Infection and Immunity, Mailman School of Public Health, Columbia University, USA. rt2249@columbia.edu
Background:
We performed a longitudinal study of viral etiology in samples collected in New York City during May 2009 to May 2010 from outpatients with fever or respiratory disease symptoms in the context of a pilot respiratory virus surveillance system.
Methods:
Samples were assessed for the presence of 13 viruses, including influenza A virus, by MassTag PCR.
Results:
At least one virus was detected in 52% of 940 samples analyzed, with 3% showing co-infections. The most frequently detected agents were rhinoviruses and influenza A, all representing the 2009 pandemic H1N1 strain. The incidence of influenza H1N1-positive samples was highest in late spring 2009, followed by a decline in summer and early fall, when rhinovirus infections became predominant before H1N1 reemerged in winter. Our study also identified a focal outbreak of enterovirus 68 in the early fall of 2009.
Conclusion:
MassTag multiplex PCR affords opportunities to track the epidemiology of infectious diseases and may guide clinicians and public health practitioners in influenza-like illness and outbreak management. Nonetheless, a substantial proportion of influenza-like illness remains unexplained underscoring the need for additional platforms.
