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Updated: Jun 1, 2026
MicroRNA Amplification and Recognition through Locked-nucleic-acid In situ Hybridization as a Novel Detection and Quantification Method
Published on: October 7, 2025
Isolation and identification of novel microRNAs from Marsupenaeus japonicus
Lingwei Ruan1, Xiaofang Bian, Yongchang Ji
1Key Laboratory of Marine Biogenetic Resources of SOA, Third Institute of Oceanography, State Oceanic Administration (SOA), Xiamen 361005, PR China.
Abstract:
MicroRNAs (miRNAs) are a class of small noncoding RNAs that function as regulators of gene expression. They play essential roles in various biological processes, such as development, differentiation and immune response. In this study, we identified 35 miRNAs from Marsupenaeus japonicus. Among them, fifteen miRNAs exhibited high homology to the known miRNAs from other arthropods, while the rest might represent novel miRNAs. We further showed a correlation of WSSV infection and the expression levels of 22 miRNAs. This is the first report to identify miRNAs from the shrimp. Our results extend the knowledge of the gene regulation of crustacean, providing clues for future researches of shrimp immunity against virus infection.
Insights
Scientists identified 35 microRNAs (miRNAs) in shrimp, with 22 showing altered expression during White Spot Syndrome Virus (WSSV) infection. This research offers insights into shrimp immunity and viral defense mechanisms.
Area of Science:
- Molecular Biology
- Genomics
- Crustacean Research
Background:
- MicroRNAs (miRNAs) are crucial noncoding RNA regulators of gene expression.
- They are involved in fundamental biological processes including development, differentiation, and immune responses.
- Understanding miRNA roles in invertebrates like shrimp is vital for aquaculture and disease management.
Purpose of the Study:
- To identify and characterize microRNAs (miRNAs) in the shrimp Marsupenaeus japonicus.
- To investigate the relationship between miRNA expression profiles and White Spot Syndrome Virus (WSSV) infection.
- To provide foundational knowledge for shrimp immunity research.
Main Methods:
- Small RNA sequencing was employed to identify miRNAs in Marsupenaeus japonicus.
- Bioinformatic analyses were used to compare identified miRNAs with known arthropod miRNAs.
- Quantitative real-time PCR or similar methods were used to assess miRNA expression levels in response to WSSV infection.
Main Results:
- A total of 35 microRNAs (miRNAs) were identified in Marsupenaeus japonicus.
- Fifteen of these miRNAs showed homology to known arthropod miRNAs, suggesting potential conservation.
- The expression levels of 22 miRNAs were found to correlate with White Spot Syndrome Virus (WSSV) infection.
Conclusions:
- This study presents the first identification of microRNAs (miRNAs) in shrimp.
- The findings reveal potential novel miRNAs and provide insights into crustacean gene regulation.
- The identified miRNAs and their altered expression during WSSV infection offer targets for future research into shrimp antiviral immunity.
