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Updated: Jun 1, 2026

Detection of Mitochondria Membrane Potential to Study CLIC4 Knockdown-induced HN4 Cell Apoptosis In Vitro
13:20

Detection of Mitochondria Membrane Potential to Study CLIC4 Knockdown-induced HN4 Cell Apoptosis In Vitro

Published on: July 17, 2018

A HIF-1 target, ATIA, protects cells from apoptosis by modulating the mitochondrial thioredoxin, TRX2

Swati Choksi1, Yong Lin, Yelena Pobezinskaya

  • 1Cell and Cancer Biology Branch, Center for Cancer Research, National Cancer Institute, National Institutes of Health, 37 Convent Drive, Bethesda, MD 20892, USA.

Molecular Cell
|June 11, 2011
PubMed

Insights

A newly identified protein, Vasn/ATIA, protects cells from apoptosis by regulating mitochondrial reactive oxygen species (ROS). This protein is crucial in glioblastoma, suggesting it as a potential diagnostic and therapeutic target.

Area of Science:

  • Cellular biology
  • Molecular mechanisms of apoptosis
  • Cancer research

Background:

  • Apoptosis regulation is vital for tissue homeostasis and cancer prevention.
  • Reactive oxygen species (ROS) play a significant role in apoptosis.
  • Hypoxia and TNFα are key inducers of apoptosis.

Purpose of the Study:

  • To identify and characterize novel regulators of apoptosis.
  • To investigate the role of Vasn/ATIA in cellular apoptosis.
  • To explore ATIA's potential as a diagnostic and therapeutic target in glioblastoma.

Main Methods:

  • Generation and analysis of ATIA knockout mice.
  • Investigation of mitochondrial antioxidant function and ROS generation.
  • Analysis of ATIA expression in human glioblastoma samples.
  • Assessment of ATIA knockdown effects on glioblastoma cell apoptosis.

Main Results:

  • Vasn/ATIA was identified as a HIF-1 target that protects against TNFα- and hypoxia-induced apoptosis.
  • ATIA regulates mitochondrial thioredoxin-2 function and ROS levels.
  • ATIA is highly expressed in human glioblastoma.
  • ATIA knockdown sensitizes glioblastoma cells to hypoxia-induced apoptosis.

Conclusions:

  • ATIA is a HIF-1 target involved in regulating mitochondrial redox pathways.
  • ATIA plays a protective role against apoptosis.
  • ATIA represents a potential diagnostic marker and therapeutic target for glioblastoma.

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