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Purified autoantibodies against glucose-regulated protein 78 (GRP78) promote apoptosis and decrease invasiveness of

Marie Cohen1, Patrick Petignat

  • 1Department of Gynecology-Obstetrics, 30 boulevard de la Cluse, Faculty of Medicine, Geneva 14, Switzerland. marie.cohen@hcuge.ch

Cancer Letters
|June 11, 2011
PubMed

Insights

Autoantibodies against glucose-regulated protein 78 (GRP78) may serve as ovarian cancer biomarkers. Purified GRP78 autoantibodies reduced ovarian cancer cell invasion and increased apoptosis, suggesting a protective role.

Area of Science:

  • Oncology
  • Immunology
  • Biochemistry

Background:

  • Circulating glucose-regulated protein 78 (GRP78) autoantibodies are potential biomarkers for epithelial ovarian cancer.
  • Limited research exists on the identification and role of these autoantibodies in ovarian cancer development.

Purpose of the Study:

  • To characterize GRP78 autoantibodies found in ovarian cancer patients.
  • To investigate the role of these autoantibodies in ovarian tumor development.

Main Methods:

  • Purification of GRP78 autoantibodies from the sera of ovarian cancer patients.
  • Testing the effects of purified GRP78 autoantibodies on the SKOV-3 ovarian cancer cell line.

Main Results:

  • Treatment with GRP78 autoantibodies led to a decrease in the invasion of SKOV-3 ovarian cancer cells.
  • An increase in hydrogen peroxide (H2O2)-induced apoptosis was observed in SKOV-3 cells upon exposure to GRP78 autoantibodies.

Conclusions:

  • GRP78 autoantibodies may play a protective role against ovarian cancer progression.
  • Further research into GRP78 autoantibodies could lead to novel diagnostic and therapeutic strategies for ovarian cancer.

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