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Related Concept Videos

Methods for Studying Drug Absorption: In vitro01:16

Methods for Studying Drug Absorption: In vitro

In vitro experiments are crucial for understanding the transport and absorption of drugs through biological materials. These studies employ varied methods such as the diffusion cell method, the everted sac technique, and the everted ring technique.
The diffusion cell method uses a two-compartment cell, including a donor compartment with the drug solution, which simulates the environment where the drug is applied, and a receptor compartment with a buffer solution, which simulates the environment...
Methods for Studying Drug Absorption: In situ01:09

Methods for Studying Drug Absorption: In situ

In situ experiments, such as the Doluisio method and Single-Pass Perfusion technique, provide critical insights into drug uptake by simulating in vivo conditions for drug absorption.
The Doluisio method involves perfusing a prepared segment of a rat's small intestine with a solution of radiolabeled drug and a non-absorbable marker. This helps to differentiate between absorbed and non-absorbed drug concentrations. The intestinal segment is connected at both ends using tubing and syringes,...
Measurement of Bioavailability: Pharmacokinetic Methods01:30

Measurement of Bioavailability: Pharmacokinetic Methods

Pharmacokinetics is a vital branch of pharmacology that examines how drugs are absorbed, distributed, metabolized, and excreted by the body. Two key methodologies in pharmacokinetics are plasma drug concentration studies and urinary drug excretion analyses, both of which provide critical insights into a drug's therapeutic efficacy and bioavailability.Plasma Drug Concentration-Time StudiesPlasma drug concentration-time studies involve analyzing blood samples at specific intervals to quantify...
Measurement of Bioavailability: Pharmacodynamic Methods01:20

Measurement of Bioavailability: Pharmacodynamic Methods

Pharmacodynamic methods provide insights into a drug's effects on physiological processes over time and play a crucial role in understanding bioavailability and therapeutic efficacy. These methods can be broadly classified into acute pharmacological and therapeutic response approaches, each with distinct mechanisms and applications.The acute pharmacological response method directly correlates a drug's physiological effects, such as ECG or pupil diameter changes, to its time course in the body.
Microbial Growth Measurement: Indirect Methods01:27

Microbial Growth Measurement: Indirect Methods

Estimating microbial growth is essential for understanding population dynamics and environmental adaptations. Indirect methods provide valuable insights by measuring parameters such as turbidity, metabolic activity, and biomass, enabling efficient and reproducible assessments.During exponential growth, microbial cells scatter light proportionally to their biomass, a principle used in turbidity measurements. About one million cells per milliliter produce detectable scattering, which a...
Drug Dosing in Renal Diseases: Measurement of Glomerular Filtration Rate01:25

Drug Dosing in Renal Diseases: Measurement of Glomerular Filtration Rate

The glomerular filtration rate (GFR) is a critical indicator of kidney health, reflecting how well the kidneys filter blood. Changes in GFR can signal potential kidney impairment, necessitating accurate measurement methods to monitor kidney function effectively.Various molecules can serve as markers for GFR measurement, with the ideal marker meeting several specific criteria. It must freely filter at the glomerulus, avoid reabsorption or secretion by the renal tubules, remain unmetabolized, not...

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Related Experiment Video

Updated: Jun 1, 2026

An In Vitro Batch-culture Model to Estimate the Effects of Interventional Regimens on Human Fecal Microbiota
07:15

An In Vitro Batch-culture Model to Estimate the Effects of Interventional Regimens on Human Fecal Microbiota

Published on: July 31, 2019

[Measurement of inulin: development].

Pierre Delanaye1, Marie Souvignet, Laurence Dubourg

  • 1Service de néphrologie-dialyse-transplantation rénale, Université de Liège, CHU Sart Tilman, Liège, Belgique.

Annales De Biologie Clinique
|June 11, 2011
PubMed
Summary

Measuring glomerular filtration rate (GFR) using inulin clearance is complex and prone to interferences. Various methods exist for inulin measurement, each with unique advantages and limitations requiring further validation.

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A High-throughput Method for Measurement of Glomerular Filtration Rate in Conscious Mice
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A High-throughput Method for Measurement of Glomerular Filtration Rate in Conscious Mice

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Last Updated: Jun 1, 2026

An In Vitro Batch-culture Model to Estimate the Effects of Interventional Regimens on Human Fecal Microbiota
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Published on: July 31, 2019

Extraction and Quantification of Soluble, Radiolabeled Inositol Polyphosphates from Different Plant Species using SAX-HPLC
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A High-throughput Method for Measurement of Glomerular Filtration Rate in Conscious Mice
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A High-throughput Method for Measurement of Glomerular Filtration Rate in Conscious Mice

Published on: May 10, 2013

Area of Science:

  • Nephrology
  • Biochemistry
  • Analytical Chemistry

Context:

  • Glomerular filtration rate (GFR) is a key indicator of kidney function.
  • Inulin clearance is the established gold standard for GFR measurement.
  • Accurate inulin quantification in biological samples remains challenging.

Purpose:

  • To review and compare existing methods for inulin measurement in urine and plasma.
  • To highlight the complexities and potential interferences associated with inulin assays.
  • To identify the need for further analytical validation of available inulin measurement techniques.

Summary:

  • Inulin clearance, despite being the gold standard for GFR, involves complex measurement techniques.
  • Common methods include "acid" colorimetric assays (measuring fructose) and enzymatic methods.
  • High-performance liquid chromatography (HPLC) offers specificity and accuracy, but further validation of all methods is needed.

Impact:

  • Improved understanding of the analytical challenges in GFR assessment.
  • Guidance for selecting appropriate inulin measurement methods in clinical and research settings.
  • Highlights the necessity for rigorous validation to ensure reliable GFR determination.