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[Evaluation of the analyzer Glycomat 3000 (Biocode Hycel) for HbA1c]
Laetitia Motte1, Emmanuelle Guillard, Stéphane Jaisson
1Laboratoire de biologie et recherche pediatriques, CHU de Reims.
Abstract:
HbA(1c) represents a key parameter in the monitoring of glycemic balance in diabetic patients. It may be assayed by various methods, among which high-pressure liquid chromatography (HPLC). We have evaluated a new HPLC analyzer, Glycomat 3000 (Biocode Hycel). HbA(1c) values are well correlated (r(2) = 0.994) with those obtained by the HPLC analyzer used routinely in our laboratory (Variant II, Bio-Rad). However, the precision of assays can be at the limit of acceptability (CV of repeatability between 0.7 and 1.4% and CV of reproducibility between 3.3 and 4.1%) if strict conditions of calibration and quality control are not implemented. The assay is sensitive to the interference of modified fractions of hemoglobin (labile HbA(1c) and carbamylated Hb) in the clinical range, and to the presence of HbF. Besides, Glycomat 3000 is a friendly and easy to use analyzer.
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