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Updated: Jun 1, 2026

Identification of Homologous Recombination Events in Mouse Embryonic Stem Cells Using Southern Blotting and Polymerase Chain Reaction
Published on: November 20, 2018
Characterization of meiotic recombination initiation sites using pulsed-field gel electrophoresis
Sarah Farmer1, Wing-Kit Leung, Hideo Tsubouchi
1MRC Genome Damage and Stability Centre, University of Sussex, Sussex, UK. s.farmer@sussex.ac.uk
Abstract:
High levels of homologous recombination are induced during meiosis. This meiotic recombination is initiated by programmed formation of DNA double-strand breaks (DSBs) by a conserved meiosis-specific protein, Spo11. Meiotic DSBs are not formed at random along chromosomes but are formed in clusters known as recombination hot spots. To understand the regulation of this initiation step of meiotic recombination, determining the timing and location of meiotic DSBs is essential. In this chapter, we describe a method to detect genome-wide meiotic DSBs by using a combination of pulsed-field gel electrophoresis and Southern blotting.
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