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SNP-based mapping of crossover recombination in Caenorhabditis elegans

Grace C Bazan1, Kenneth J Hillers

  • 1Biological Sciences, California Polytechnic State University, San Luis Obispo, CA 3407-0401, USA. gracebazan@gmail.com

Methods in Molecular Biology (Clifton, N.J.)
|June 11, 2011
PubMed

Abstract:

Caenorhabditis elegans is an important experimental organism for the study of recombination during meiosis. Here, we provide methods for the use of single-nucleotide polymorphisms (SNPs) for the study of crossing over in C. elegans.

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Because the DNA segments are cut and reorganized in a direction-specific manner, site-specific recombination has emerged as an efficient genetic engineering technique. Flippase and Cyclization recombinases or Flp and Cre, respectively, are two members of the tyrosine recombinase family derived from bacteriophages, that are used to mediate site-specific DNA insertions, deletions, and targeted expression of proteins in mammalian cell lines.
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