Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Dehydration-induced expression of a 31-kDa dehydrin in Polypodium polypodioides (Polypodiaceae) may enable large, reversible deformation of cell walls.

American journal of botany·2011

Same author

Determination of the forces imposed by micro and nanopipettes during DOPC: DOPS liposome manipulation.

Chemistry and physics of lipids·2009

Same author

Cytoskeleton-membrane interactions in neuronal growth cones: a finite analysis study.

Journal of biomechanical engineering·2008

Same author

Parallel force measurement with a polymeric microbeam array using an optical microscope and micromanipulator.

Computer methods and programs in biomedicine·2008

Same author

Collagen's triglycine repeat number and phylogeny suggest an interdomain transfer event from a Devonian or Silurian organism into Trichodesmium erythraeum.

Journal of molecular evolution·2008

Same author

The effect of deformation on room temperature Coulomb blockade using conductive carbon nanotubes.

Annual International Conference of the IEEE Engineering in Medicine and Biology Society. IEEE Engineering in Medicine and Biology Society. Annual International Conference·2007

Search research articles

Related Experiment Video

Updated: Jun 1, 2026

Immunodetection of Outer Membrane Proteins by Flow Cytometry of Isolated Mitochondria

Immunodetection of Outer Membrane Proteins by Flow Cytometry of Isolated Mitochondria

Published on: September 18, 2014

Atomic force microscopy of isolated mitochondria.

Bradley E Layton¹, M Brent Boyd

¹Applied Computing and Electronics, The University of Montana College of Technology, Missoula, MT, USA. layton@coe.drexel.edu

Methods in Molecular Biology (Clifton, N.J.)

|June 11, 2011

Summary

This study details methods for imaging mitochondria using atomic force microscopy, revealing outer membrane pores in organelles challenged by glucose or dinitrobenzene (DNB). The focus is on the imaging techniques for these vital cellular components.

More Related Videos

Mitochondria and Endoplasmic Reticulum Imaging by Correlative Light and Volume Electron Microscopy

Mitochondria and Endoplasmic Reticulum Imaging by Correlative Light and Volume Electron Microscopy

Published on: July 20, 2019

Analysis of Brain Mitochondria Using Serial Block-Face Scanning Electron Microscopy

Analysis of Brain Mitochondria Using Serial Block-Face Scanning Electron Microscopy

Published on: July 9, 2016

Related Experiment Videos

Last Updated: Jun 1, 2026

Immunodetection of Outer Membrane Proteins by Flow Cytometry of Isolated Mitochondria

Immunodetection of Outer Membrane Proteins by Flow Cytometry of Isolated Mitochondria

Published on: September 18, 2014

Mitochondria and Endoplasmic Reticulum Imaging by Correlative Light and Volume Electron Microscopy

Mitochondria and Endoplasmic Reticulum Imaging by Correlative Light and Volume Electron Microscopy

Published on: July 20, 2019

Analysis of Brain Mitochondria Using Serial Block-Face Scanning Electron Microscopy

Analysis of Brain Mitochondria Using Serial Block-Face Scanning Electron Microscopy

Published on: July 9, 2016

Area of Science:

Cell Biology
Biophysics
Toxicology

Background:

Mitochondria are crucial for cellular energy production and are affected by metabolic and toxic challenges.
Understanding mitochondrial structure, particularly membrane pores, is key to comprehending cellular dysfunction.

Purpose of the Study:

To describe detailed methods for isolating and imaging metabolically and toxicologically challenged mitochondria.
To showcase the capabilities of atomic force microscopy in visualizing intact organelle structures.

Main Methods:

Isolation of mitochondria from rat dorsal root ganglia or brain.
Exposure of isolated mitochondria to glucose or dinitrobenzene (DNB) to simulate specific cellular conditions.
Imaging of mitochondria using atomic force microscopy (AFM) to visualize membrane structures.

Main Results:

Atomic force microscopy provided high-resolution images of intact mitochondria.
Specific images revealed outer membrane structures, including voltage-dependent, anion-selective channel pores.
The study includes images of isolated nuclei and notes on common pitfalls in organelle isolation and imaging.

Conclusions:

Detailed imaging methods for challenged mitochondria using AFM are presented.
The techniques allow visualization of fine membrane structures on intact organelles.
This work provides a methodological foundation for further studies on mitochondrial function and dysfunction.