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Restriction endonuclease analysis of Eikenella corrodens
C K Chen1, G J Sunday, J J Zambon
1Department of Oral Biology, State University of New York, Buffalo School of Dental Medicine 14214.
Journal of Clinical Microbiology
|June 1, 1990
Summary
Eikenella corrodens exhibits significant genetic diversity, with many strains showing unique genetic patterns. Restriction endonuclease analysis (REA) reveals multiple E. corrodens clones within single individuals, suggesting complex transmission dynamics.
Area of Science:
- Oral microbiology
- Bacterial genetics
- Infectious disease epidemiology
Background:
- Eikenella corrodens is a common oral bacterium with unclear links to periodontitis.
- This species is known for its phenotypic diversity.
- Extraoral infections highlight its pathogenic potential.
Purpose of the Study:
- To assess the genetic diversity of Eikenella corrodens using restriction endonuclease analysis (REA).
- To explore the utility of REA in understanding E. corrodens transmission.
Main Methods:
- Restriction endonuclease analysis (REA) was performed on 87 E. corrodens isolates.
- Isolates were sourced from dental plaques and extraoral infections.
- Isolates were also collected from periodontally healthy and periodontitis patients.
Main Results:
- REA demonstrated significant genetic heterogeneity within E. corrodens.
- Most independent isolates displayed unique strain-specific restriction patterns.
- Multiple E. corrodens clones were identified within individual subjects, sometimes within a single periodontal pocket.
Conclusions:
- Eikenella corrodens is genetically diverse.
- REA is a valuable tool for investigating E. corrodens epidemiology and transmission.
- The presence of multiple clones in individuals suggests complex colonization and infection patterns.