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Related Experiment Video

Updated: Jun 1, 2026

Correlative Microscopy for 3D Structural Analysis of Dynamic Interactions

Correlative Microscopy for 3D Structural Analysis of Dynamic Interactions

Published on: June 24, 2013

Two-beam cross-correlation: a method to characterize transport phenomena in micrometer-sized structures.

M Brinkmeier¹, K Dörre, J Stephan

¹Max-Planck-Institut für biophysikalische Chemie, Am Fassberg, D-37077 Göttingen, Germany.

Analytical Chemistry

|June 14, 2011

Summary

This study introduces a fluorescence correlation spectroscopy method for precise microchannel flow analysis. The technique rapidly measures flow velocity and diffusion properties, enabling applications in chromatography and high-throughput screening.

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Area of Science:

Physics
Chemistry
Biotechnology

Background:

Accurate characterization of fluid dynamics in microstructured channels is crucial for applications like capillary electrophoresis (CE) and high-performance liquid chromatography (HPLC).
Traditional methods for measuring microflow properties can be time-consuming or lack the required spatial resolution.

Purpose of the Study:

To develop and validate an experimental method for determining flow velocity and angle in microstructured channels.
To enable simultaneous measurement of diffusion properties of single molecules.

Main Methods:

Utilized fluorescence correlation spectroscopy (FCS) with two micrometer-sized, spatially separated volume elements.
Recorded and mathematically evaluated the cross-correlation signal between these elements.
Implemented a two-beam cross-correlation approach for enhanced data acquisition.

Main Results:

Achieved fast and easy determination of flow velocities as low as 200 μm/s.
Enabled simultaneous measurement of diffusion properties with a detection time of 5-100 s and <20% error.
Obtained vectorial flow data with spatial resolution of 1-2 μm.

Conclusions:

The developed two-beam cross-correlation FCS method offers a precise, simple, and fast approach for microflow analysis.
This technique has significant potential for applications in CE, HPLC, chemical kinetics, and high-throughput screening.