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On-line solid-phase extraction of triazine herbicides using a molecularly imprinted polymer for selective sample
B Bjarnason1, L Chimuka, O Ramström
1Department of Analytical Chemistry and Department of Pure and Applied Biochemistry, Lund University, Box 124, S-221 00 Lund, Sweden.
Abstract:
A coupled-column system, consisting of a combination of a molecularly imprinted polymer (MIP) and a C(18)-silica column, was used for selective triazine detection in the HPLC mode. Complex aqueous samples, spiked with triazines, were selectively extracted by the MIP followed by subsequent identification by analytical reversed-phase chromatography. The MIP showed good performance for selectively discriminating triazines from humic acid, whereas urine and apple extract had some tendency to be retained by the MIP. Enrichment was observed in all cases, and triazine-enrichment factors of up to 100 could be recorded, with good extraction efficiency (74-77%). The results indicate that the high selectivity of MIPs can be favorably used for selective sample enrichment in conjunction with reversed-phase HPLC.
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