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In Situ Characterization of Shewanella oneidensis MR1 Biofilms by SALVI and ToF-SIMS
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In situ, High-Resolution Measurement of Dissolved Sulfide Using Diffusive Gradients in Thin Films with

P R Teasdale1, S Hayward, W Davison

  • 1Environmental Science, IENS, Lancaster University, Lancaster LA1 4YQ, U.K.

Analytical Chemistry
|June 14, 2011
PubMed
Summary
This summary is machine-generated.

Diffusive gradients in thin films (DGT) effectively measures dissolved sulfide in water. This technique uses a gel to accumulate sulfide, which is then quantified using colorimetry or densitometry.

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Area of Science:

  • Environmental Chemistry
  • Analytical Chemistry
  • Geochemistry

Background:

  • Dissolved sulfide is a critical analyte in aquatic environments.
  • Accurate measurement of sulfide is essential for environmental monitoring.
  • Existing methods for sulfide measurement can be complex or have limitations.

Purpose of the Study:

  • To develop and validate the diffusive gradients in thin films (DGT) technique for dissolved sulfide measurement.
  • To assess the performance of DGT in synthetic and natural aquatic samples.
  • To evaluate DGT's capability for high-resolution depth profiling.

Main Methods:

  • Utilized diffusive gradients in thin films (DGT) with a polyacrylamide hydrogel and silver iodide (AgI).
  • Sulfide diffused through the gel and reacted with AgI to form silver sulfide (Ag2S).
  • Quantified accumulated sulfide via purge-and-trap colorimetry or scanner-based densitometry.

Main Results:

  • DGT accurately determined sulfide concentrations in synthetic solutions with 95% recovery.
  • Accumulated sulfide mass showed an inverse relationship with diffusion-layer thickness.
  • The densitometric method achieved a detection limit of 0.13 μmol L⁻¹ for a 24-h deployment.
  • DGT provided reliable sulfide measurements in anoxic lacustrine waters and submillimeter depth profiles in sediments.

Conclusions:

  • DGT is a robust and accurate technique for measuring dissolved sulfide in various aquatic environments.
  • The method offers high sensitivity and spatial resolution for sulfide analysis.
  • DGT provides a valuable tool for studying sulfide dynamics in environmental samples.