Continuous-Flow and Flow Injection pH Gradients for Spectrophotometric Determinations of Mixtures of Nucleic Acid...

Area of Science:

• Analytical Chemistry
• Biochemistry
• Spectrophotometry

Background:

• Accurate quantification of nucleic acid components in complex mixtures is crucial for various scientific applications.
• Traditional methods can be time-consuming and susceptible to interference.
• Development of rapid and robust analytical techniques is needed.

Purpose of the Study:

• To propose a novel procedure for the rapid determination of nucleic acid component mixtures.
• To develop and validate continuous-flow and flow injection systems with on-line pH gradients.
• To enable accurate quantification in the presence of interferences using minimal standards.

Main Methods:

• Utilized spectrophotometric multivariate data analysis.
• Developed three flow systems generating on-line pH gradients from acidic and basic titrants.
• Employed alternating least squares multivariate curve resolution for quantitative analysis.
• Validated methods with synthetic and real sample mixtures.

Main Results:

• The proposed methods demonstrated rapid determination of nucleic acid component mixtures.
• Quantitative analysis was accurate even in the presence of interfering substances.
• The procedure requires only a single pure standard for each analyte.
• Validated methods showed results concordant with labeled values, with relative prediction errors around 5%.

Conclusions:

• The developed flow systems and multivariate analysis provide a rapid and accurate method for nucleic acid component determination.
• The approach effectively handles complex mixtures and interferences.
• This technique offers a significant advancement in the analysis of nucleic acid samples.