Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

UV–Vis Spectrometers

UV–Vis Spectrometers

The absorbance of UV and visible (UV–visible) radiations is measured using a UV–visible spectrophotometer. Deuterium lamps, which emit UV radiation, and tungsten lamps, which produce radiation in the visible region, are used as light sources in UV–visible spectrophotometers. A monochromator or prism is used for diffraction grating, i.e., to split the incoming radiation into different wavelengths. A system of slits is used to focus the desired wavelength on the sample cell. Samples for...

Spectrophotometry: Introduction

Spectrophotometry: Introduction

Spectrophotometry is the quantitative measurement of the absorption, reflection, diffraction, or transmission of electromagnetic radiation through a material as a function of the intensity and wavelength of the radiation. A spectrophotometer is a device used to measure the change in the radiation intensity caused by its interaction with the material.
The essential components of a spectrophotometer include a source of electromagnetic radiation, a slot for placing a material to be analyzed, and a...

Atomic Spectroscopy: Absorption, Emission, and Fluorescence

Atomic Spectroscopy: Absorption, Emission, and Fluorescence

Atomic spectroscopy is a vital tool in elemental analysis, both qualitatively and quantitatively. It can be broadly divided into optical spectroscopy, mass spectroscopy, and X-ray spectroscopy methods. The optical spectroscopic methods are atomic absorption spectroscopy (AAS), atomic emission spectroscopy (AES), and atomic fluorescence spectroscopy (AFS). The first step in all three methods is atomization, where the solid, liquid, or solution-phase samples are converted into gas-phase atoms and...

Atomic Absorption Spectroscopy: Interference

Atomic Absorption Spectroscopy: Interference

Interference leads to systematic error in atomic absorption (AA) measurements by enhancing or diminishing the analytical signal or the background. These interferences can be grouped into three main categories: spectral interference, chemical interference, and physical interference.
Spectral interference occurs when signals from other elements or molecules overlap with the analyte signal, falsely elevating or masking the analyte's absorbance. This interference can be corrected using Zeeman,...

Atomic Absorption Spectroscopy: Instrumentation

Atomic Absorption Spectroscopy: Instrumentation

An atomic absorption spectrophotometer (AAS) comprises several components: a radiation source, an atomizer, a monochromator, and a detector. The radiation source can be a hollow-cathode lamp (HCL) or an electrodeless-discharge lamp (EDL), both of which provide a narrow emission line of the required wavelength. However, some instruments use continuum sources and high-resolution monochromators to achieve a narrow range of radiation.
The atomizer used in AAS can be either a flame atomizer or an...

Ultraviolet and Visible (UV–Vis) Spectroscopy: Overview

Ultraviolet and Visible (UV–Vis) Spectroscopy: Overview

Ultraviolet–visible (UV–visible or UV–Vis) spectroscopy is an analytical technique that investigates the interaction between matter and UV–Vis light within the electromagnetic spectrum. This method is widely used for its versatility, simplicity, and relatively quick data acquisition, making it valuable for both qualitative and quantitative analysis. When UV–Vis radiation passes through a material, molecules absorb light depending on the energy required for electronic transitions. As a result...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Effectiveness of post-trastuzumab deruxtecan treatments and incidence of interstitial lung disease in HER2-positive metastatic breast cancer: a real-world, observational cohort study.

ESMO open·2025

Same author

Metabolomic responses of an estuarine benthic amphipod to heavy metals at urban-runoff concentrations.

Water science and technology : a journal of the International Association on Water Pollution Research·2019

Same author

Enhanced expression of nidogen 1 around the nest of basal cell carcinoma compared with that around squamous cell carcinoma.

Medical molecular morphology·2018

Same author

A novel HLA-C allele, HLA-C*07:02:01:17N, with an alternative splice site.

HLA·2018

Same author

Head-to-Head Visual Comparison between Brain Perfusion SPECT and Arterial Spin-Labeling MRI with Different Postlabeling Delays in Alzheimer Disease.

AJNR. American journal of neuroradiology·2017

Same author

Long-term weight-change slope, weight fluctuation and risk of type 2 diabetes mellitus in middle-aged Japanese men and women: findings of Aichi Workers' Cohort Study.

Nutrition & diabetes·2017

Search research articles

Related Experiment Video

Updated: Jun 1, 2026

Multicolor Fluorescence Detection for Droplet Microfluidics Using Optical Fibers

Multicolor Fluorescence Detection for Droplet Microfluidics Using Optical Fibers

Published on: May 5, 2016

Diffractive optical chemical sensor based on light absorption.

F Nakajima¹, Y Hirakawa, T Kaneta

¹Department of Chemical Systems and Engineering, Graduate School of Engineering, Kyushu University, Hakozaki, Fukuoka 812, Japan.

Analytical Chemistry

|June 14, 2011

Summary

This summary is machine-generated.

A novel chemical sensor utilizes light absorption and beam diffraction for pH detection. This gelatin film sensor with thymolphthalein shows promise for sensitive and accurate measurements.

More Related Videos

$Synthesis and Operation of Fluorescent-core Microcavities for Refractometric Sensing$

Synthesis and Operation of Fluorescent-core Microcavities for Refractometric Sensing

Published on: March 13, 2013

Novel Techniques for Observing Structural Dynamics of Photoresponsive Liquid Crystals

Novel Techniques for Observing Structural Dynamics of Photoresponsive Liquid Crystals

Published on: May 29, 2018

Related Experiment Videos

Last Updated: Jun 1, 2026

Multicolor Fluorescence Detection for Droplet Microfluidics Using Optical Fibers

Multicolor Fluorescence Detection for Droplet Microfluidics Using Optical Fibers

Published on: May 5, 2016

$Synthesis and Operation of Fluorescent-core Microcavities for Refractometric Sensing$

Synthesis and Operation of Fluorescent-core Microcavities for Refractometric Sensing

Published on: March 13, 2013

Novel Techniques for Observing Structural Dynamics of Photoresponsive Liquid Crystals

Novel Techniques for Observing Structural Dynamics of Photoresponsive Liquid Crystals

Published on: May 29, 2018

Area of Science:

Analytical Chemistry
Materials Science
Optics

Background:

Chemical sensors are crucial for various applications, including environmental monitoring and diagnostics.
Existing methods like fluorometry have limitations in sensitivity or complexity.
Developing new sensing mechanisms is essential for advancing analytical capabilities.

Purpose of the Study:

To propose and investigate a new chemical sensor based on light absorption and beam diffraction.
To evaluate the sensor's performance using the pH indicator thymolphthalein in a gelatin film.
To compare the sensitivity of this novel method with existing techniques like fluorometry.

Main Methods:

Fabrication of a gelatin film with an array of zones containing thymolphthalein.
Exposure of the film to solutions with varying pH (acidic to alkaline).
Observation of color change and subsequent use of the film as a transmission grating for laser beam diffraction.

Main Results:

A distinct blue stripe appeared in the gelatin film upon transition from acidic to alkaline conditions.
The blue stripe effectively functioned as a transmission grating, diffracting an introduced laser beam.
Theoretical analysis indicates the method's sensitivity is comparable to or exceeds that of fluorometry.

Conclusions:

A novel, light absorption/beam diffraction-based chemical sensor for pH detection has been successfully proposed.
The sensor demonstrates potential for high sensitivity, comparable to or better than fluorometric methods.
This approach offers a new avenue for developing advanced chemical sensing technologies.