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Related Concept Videos

Flow Cytometry01:23

Flow Cytometry

The development of flow cytometry techniques began in 1934 with initial attempts by Andrew Moldavan, a bacteriologist who counted the cells in a flowing capillary system. Moldavan pumped cells through a capillary tube focused under a microscope for visualization. The invention of photometry allowed the measurement of differentially-stained cells, and Louis Kamentsky developed the first multiparameter flow cytometer in 1965 to identify and count the cancer cells in cervical tissue specimens.
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Related Experiment Video

Updated: Jun 1, 2026

Microfluidic Platform with Multiplexed Electronic Detection for Spatial Tracking of Particles
11:54

Microfluidic Platform with Multiplexed Electronic Detection for Spatial Tracking of Particles

Published on: March 13, 2017

Microchip flow cytometry using electrokinetic focusing.

D P Schrum1, C T Culbertson, S C Jacobson

  • 1Oak Ridge National Laboratory, P.O. Box 2008, Oak Ridge, Tennessee 37831-6142.

Analytical Chemistry
|June 14, 2011
PubMed
Summary
This summary is machine-generated.

This study demonstrates a microfluidic device for flow cytometry, enabling particle detection and counting using laser light scattering and fluorescence. The system successfully distinguished between different particle sizes and analyzed mixtures, achieving high throughput.

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Area of Science:

  • Microfluidics
  • Analytical Chemistry
  • Biotechnology

Background:

  • Flow cytometry is a crucial technique for cell analysis.
  • Microfluidic devices offer miniaturization and high throughput for analytical methods.
  • Integrating flow cytometry with microfluidics presents opportunities for enhanced diagnostics.

Purpose of the Study:

  • To demonstrate flow cytometry on a microfabricated device.
  • To analyze latex particles using laser light scattering and fluorescence.
  • To assess the device's capability for particle discrimination and mixture analysis.

Main Methods:

  • Microfluidic device with a cross-intersection for electrokinetic focusing.
  • Laser light scattering and fluorescence coincidence measurements for particle detection.
  • Analysis of 1 and 2 μm latex particles, both labeled and unlabeled.

Main Results:

  • Successful detection and counting of latex particles.
  • Distinction between particle sizes (1 and 2 μm) based on scattering and fluorescence.
  • Achieved a maximum sample throughput of 34 particles/s.
  • Accurate analysis of mixtures with varying proportions of labeled and unlabeled particles.

Conclusions:

  • The microfabricated device is effective for flow cytometry applications.
  • The system enables precise particle analysis and quantification.
  • This technology holds promise for miniaturized, high-throughput particle analysis.