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Packed column supercritical fluid chromatography/mass spectrometry for high-throughput analysis. Part 2
M C Ventura1, W P Farrell, C M Aurigemma
1Alanex Corporation, 3550 General Atomics Court, San Diego, California 92121-1194.
Analytical Chemistry
|June 14, 2011
Summary
Supercritical fluid chromatography-mass spectrometry (SFC/MS) offers significant advantages over liquid chromatography-mass spectrometry (LC/MS) for high-throughput analysis. This advanced SFC/MS system enhances efficiency, precision, and solvent compatibility.
Area of Science:
- Analytical Chemistry
- Chromatography
- Mass Spectrometry
Background:
- Previous work established supercritical fluid chromatography-mass spectrometry (SFC/MS) using negative-mode atmospheric-pressure chemical ionization (APCI) for high-throughput applications.
- Liquid chromatography-mass spectrometry (LC/MS) is a common technique but can be limited by throughput and solvent compatibility.
Purpose of the Study:
- To extend previous SFC/MS research by demonstrating its effectiveness using positive ion APCI for analyzing diverse compounds.
- To evaluate the benefits of SFC/MS, including time savings, improved chromatographic efficiency, and enhanced quantitation.
- To assess the utility of SFC/MS for flow injection analysis (FIA) and compare solvent compatibility and cycle times with LC/MS.
Main Methods:
- Interfacing a supercritical fluid chromatograph to a mass spectrometer (SFC/MS).
- Utilizing positive ion atmospheric-pressure chemical ionization (APCI) for compound analysis.
- Comparing SFC/MS performance against LC/MS for sample mixtures and flow injection analysis.
Main Results:
- SFC/MS with positive ion APCI effectively analyzes compounds across a wide polarity range.
- Substitution of SFC/MS for LC/MS leads to significant time savings, increased chromatographic efficiency, and more precise quantitation.
- The SFC/MS system demonstrates broader solvent compatibility and reduced sample carryover/cycle times compared to LC/MS, benefiting FIA.
Conclusions:
- SFC/MS, particularly with positive ion APCI, is a powerful technique for high-throughput analysis of diverse compounds.
- SFC/MS offers substantial improvements in speed, efficiency, precision, and solvent flexibility over traditional LC/MS.
- The described instrumental setup allows for seamless switching between LC/MS and SFC/MS modes, enhancing analytical versatility.
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