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Updated: Jun 1, 2026

Optical Trapping of Plasmonic Nanoparticles for In Situ Surface-Enhanced Raman Spectroscopy Characterizations

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Single-Microparticle Measurements: Laser Trapping-Absorption Microspectroscopy under Solution-Flow Conditions.

H B Kim¹, O Kogi, N Kitamura

¹Division of Chemistry, Graduate School of Science, Hokkaido University, Sapporo 060-0810, Japan.

Analytical Chemistry

|June 14, 2011

Summary

A novel laser trapping-microspectroscopy system enables single microparticle analysis. This technique determined Rhodamine B adsorption rates on individual microparticles for the first time.

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Area of Science:

Analytical Chemistry
Optical Physics
Biophysics

Background:

Single microparticle analysis is crucial for understanding complex processes.
Existing methods often lack the precision to isolate and study individual particles.
Laser trapping offers a non-invasive method for microparticle manipulation.

Purpose of the Study:

To develop and validate a laser trapping-microspectroscopy system for single microparticle analysis.
To investigate the forces governing microparticle trapping under fluid flow.
To optimize optical conditions for microspectroscopy of trapped particles.
To measure dye adsorption kinetics on individual microparticles.

Main Methods:

A 1064-nm laser was used for trapping single microparticles within a flow cell.
A fluid manifold system was employed to isolate the trapped particle.
The positional displacement of the trapped particle was analyzed concerning flow rate and particle size.
Absorption microspectroscopy was performed on the laser-trapped particle.
Time-course dye adsorption experiments were conducted.

Main Results:

The system successfully isolated and manipulated single microparticles.
The balance between gradient (F(g)) and Stokes (F(s)) forces determined the trapped particle's position.
Optical requirements for microspectroscopy of trapped particles were optimized.
The adsorption rate of Rhodamine B on individual microparticles was quantified for the first time.

Conclusions:

The developed laser trapping-microspectroscopy system is effective for single microparticle analysis.
This technique provides new insights into microparticle interactions and adsorption kinetics.
The study sets a precedent for real-time, single-particle adsorption measurements.