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Published on: June 13, 2021
High-performance membrane chromatography of small molecules
A Podgornik1, M Barut, J Jančar
1BIA Separations d.o.o., Teslova 30, SI-1000 Ljubljana, Slovenia.
Analytical Chemistry
|June 14, 2011
Summary
High-performance membrane chromatography (HPMC) effectively separates small molecules like peptides and oligonucleotides. This study demonstrates HPMC
Area of Science:
- Biochemistry
- Analytical Chemistry
- Separation Science
Background:
- High-performance membrane chromatography (HPMC) is established for protein and plasmid DNA separations.
- Limited research exists on HPMC applications for small molecule purification.
Purpose of the Study:
- To explore gradient and isocratic HPMC for small molecule separation using various chemistries.
- To elucidate the separation mechanisms involved in HPMC of small molecules.
Main Methods:
- Utilized Convective Interaction Media (CIM) disks with different surface chemistries.
- Investigated both gradient and isocratic HPMC conditions.
- Analyzed separation efficiency and resolution for various small molecules.
Main Results:
- Achieved efficient separation of oligonucleotides and peptides via ion-exchange HPMC.
- Successfully separated small hydrophobic molecules using reversed-phase HPMC.
- Demonstrated comparable peak resolution in both gradient and isocratic HPMC modes.
Conclusions:
- HPMC is a versatile technique applicable to diverse small molecule separations.
- Ion-exchange and reversed-phase modes are effective for specific small molecule classes.
- Gradient and isocratic HPMC offer similar resolution, providing flexibility in method development.
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