Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Search research articles

Related Experiment Video

Updated: Jun 1, 2026

Measuring Trans-Plasma Membrane Electron Transport by C2C12 Myotubes

Measuring Trans-Plasma Membrane Electron Transport by C2C12 Myotubes

Published on: May 4, 2018

Superoxide Dismutase Activity Measurement Using Cytochrome c-Modified Electrode.

F Lisdat¹, B Ge, E Ehrentreich-Förster

¹Institute of Biochemistry and Molecular Physiology, University of Potsdam, Im Biotechnologiepark, 14943 Luckenwalde, Germany, and Max Delbrück Centrum für molekulare Medizin, Robert-Rössle Strasse 10, 13122 Berlin, Germany.

Analytical Chemistry

|June 14, 2011

Summary

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Application and significance of precise computed tomography angiography (CTA) scanning technology in the assessment of lower extremity arterial diseases.

Clinical radiology·2025

Same author

Qualitative and quantitative protease activity tests based on protein degradation in three-dimensional structures.

Bioelectrochemistry (Amsterdam, Netherlands)·2024

Same author

Quantitative determination of dopamine in the presence of interfering substances supported by machine learning tools.

Bioelectrochemistry (Amsterdam, Netherlands)·2024

Same author

Photoelectrochemistry of a photosystem I - Ferredoxin construct on ITO electrodes.

Bioelectrochemistry (Amsterdam, Netherlands)·2023

Same author

Case report: human early embryonic arrest in a consanguineous Chinese family caused by a novel missense variant of PADI6.

QJM : monthly journal of the Association of Physicians·2023

Same author

Current limits of structural biology: The transient interaction between cytochrome <i>c</i> <sub></sub> and photosystem I.

Current research in structural biology·2021

A novel gold electrode method accurately detects low superoxide dismutase (SOD) activity, even within liposomes. This biosensor offers a stable and sensitive alternative for SOD quantification.

Area of Science:

Electrochemistry
Biomedical Engineering
Enzyme Assays

Background:

Superoxide dismutase (SOD) is a crucial antioxidant enzyme.
Accurate quantification of SOD activity is essential for various biological and medical studies.
Existing methods for SOD activity measurement can have limitations in sensitivity or application scope.

Purpose of the Study:

To develop a sensitive and rapid electrochemical method for quantifying superoxide dismutase (SOD) activity.
To assess the performance of a modified gold electrode for SOD detection.
To evaluate the applicability of the developed method for SOD activity determination in complex systems like liposomes.

Main Methods:

Utilized a cytochrome c-modified gold electrode for direct detection of superoxide radicals.

More Related Videos

Simultaneous Measurement of Superoxide/Hydrogen Peroxide and NADH Production by Flavin-containing Mitochondrial Dehydrogenases

Simultaneous Measurement of Superoxide/Hydrogen Peroxide and NADH Production by Flavin-containing Mitochondrial Dehydrogenases

Published on: February 24, 2018

Oxygen-Independent Assays to Measure Mitochondrial Function in Mammals

Oxygen-Independent Assays to Measure Mitochondrial Function in Mammals

Published on: May 19, 2023

Related Experiment Videos

Last Updated: Jun 1, 2026

Measuring Trans-Plasma Membrane Electron Transport by C2C12 Myotubes

Measuring Trans-Plasma Membrane Electron Transport by C2C12 Myotubes

Published on: May 4, 2018

Simultaneous Measurement of Superoxide/Hydrogen Peroxide and NADH Production by Flavin-containing Mitochondrial Dehydrogenases

Simultaneous Measurement of Superoxide/Hydrogen Peroxide and NADH Production by Flavin-containing Mitochondrial Dehydrogenases

Published on: February 24, 2018

Oxygen-Independent Assays to Measure Mitochondrial Function in Mammals

Oxygen-Independent Assays to Measure Mitochondrial Function in Mammals

Published on: May 19, 2023

Established steady-state superoxide concentrations under controlled calibration conditions.

Investigated and minimized interference from hydrogen peroxide and uric acid using long-chain thiols.

Validated the method by comparing results with a standard photometric test.

Main Results:

The modified electrode demonstrated rapid response to superoxide radicals.
The method achieved high sensitivity, detecting SOD activities as low as 10-200 munits/mL.
A good correlation was observed between the electrochemical method and the standard photometric assay.
The developed electrode modification proved stable for several days, with minimized interference.

Conclusions:

A cytochrome c-modified gold electrode provides a sensitive, rapid, and stable platform for SOD activity quantification.
The electrochemical method is suitable for determining SOD activity, including when entrapped within liposomes.
This approach offers a viable alternative to traditional SOD assay methods, particularly for low activity samples.