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Related Experiment Video

Updated: Jun 1, 2026

Enrichment of Bacterial Lipoproteins and Preparation of N-terminal Lipopeptides for Structural Determination by Mass Spectrometry

Enrichment of Bacterial Lipoproteins and Preparation of N-terminal Lipopeptides for Structural Determination by Mass Spectrometry

Published on: May 21, 2018

Lipoprotein sorting in bacteria.

Suguru Okuda¹, Hajime Tokuda

¹Department of Chemistry and Chemical Biology, Harvard University, Cambridge, Massachusetts 02138, USA. okuda@fas.harvard.edu

Annual Review of Microbiology

|June 14, 2011

Summary

Bacterial lipoproteins are essential cell surface proteins. The Lol system facilitates their transport to the outer membrane, crucial for bacterial cell function and integrity.

Area of Science:

Microbiology
Molecular Biology
Biochemistry

Background:

Bacterial lipoproteins are synthesized in the cytoplasm and anchored to the cytoplasmic membrane via an N-terminal lipid moiety.
Over 100 lipoproteins, performing diverse cell surface functions, are predicted in many bacteria.
Lipoproteins are vital for cell surface integrity and various cellular processes.

Purpose of the Study:

To review the biogenesis of bacterial lipoproteins.
To discuss their importance in cellular functions.
To elucidate the molecular mechanisms of lipoprotein sorting to the outer membrane.

Main Methods:

Biochemical studies
Genetic studies
Structural studies

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Using Caco-2 Cells to Study Lipid Transport by the Intestine

Using Caco-2 Cells to Study Lipid Transport by the Intestine

Published on: August 20, 2015

Separation of the Cell Envelope for Gram-negative Bacteria into Inner and Outer Membrane Fractions with Technical Adjustments for Acinetobacter baumannii

Separation of the Cell Envelope for Gram-negative Bacteria into Inner and Outer Membrane Fractions with Technical Adjustments for Acinetobacter baumannii

Published on: April 10, 2020

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Last Updated: Jun 1, 2026

Enrichment of Bacterial Lipoproteins and Preparation of N-terminal Lipopeptides for Structural Determination by Mass Spectrometry

Enrichment of Bacterial Lipoproteins and Preparation of N-terminal Lipopeptides for Structural Determination by Mass Spectrometry

Published on: May 21, 2018

Using Caco-2 Cells to Study Lipid Transport by the Intestine

Using Caco-2 Cells to Study Lipid Transport by the Intestine

Published on: August 20, 2015

Separation of the Cell Envelope for Gram-negative Bacteria into Inner and Outer Membrane Fractions with Technical Adjustments for Acinetobacter baumannii

Separation of the Cell Envelope for Gram-negative Bacteria into Inner and Outer Membrane Fractions with Technical Adjustments for Acinetobacter baumannii

Published on: April 10, 2020

Main Results:

Lipoproteins are synthesized as precursors and processed on the cytoplasmic membrane.
The Lol system (five proteins) mediates lipoprotein localization to the outer membrane in Escherichia coli.
Lipoproteins are critical for sorting other outer membrane components like lipopolysaccharides and β-barrel proteins.

Conclusions:

The biogenesis and sorting of bacterial lipoproteins are complex processes.
Efficient sorting of hydrophobic lipoproteins across the hydrophilic periplasm is essential.
Understanding lipoprotein transport is key to comprehending bacterial cell envelope organization and function.