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Slice Patch Clamp Technique for Analyzing Learning-Induced Plasticity
Published on: November 11, 2017
A time-dependent effect of caffeine upon lesion-induced plasticity
F Cabral-Miranda1, C A Serfaty, P Campello-Costa
1Programa de Neurociências, Departamento de Neurobiologia, Instituto de Biologia, Universidade Federal Fluminense, Niterói, RJ, Brazil.
Abstract:
During a critical period, unilateral retinal lesions induce rapid axonal sprouting of intact axons into denervated territories within the collicular visual layers. We investigated the effect of caffeine, a non-selective A(1) and A(2a) antagonist, upon the lesion-induced plasticity of retinotectal axons. Pigmented rats submitted to a temporal retinal lesion received either caffeine (30mg/kg, ip) or saline treatment. The anterograde tracing revealed that caffeine treatment during the critical period resulted in a clear reduction on the sprouting of ipsilateral fibers but to an amplification of the plasticity after PND21, thus revealing opposite effects depending on the developmental time window.
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