Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Insertion elements on lactococcal proteinase plasmids.

A J Haandrikman1, C van Leeuwen, J Kok

  • 1Department of Genetics, University of Groningen, Haren, The Netherlands.

Applied and Environmental Microbiology
|June 1, 1990
PubMed
Summary

Two similar DNA insertion sequence (IS) elements, ISS1W and ISS1N, were identified on Lactococcus lactis plasmids. These elements share high nucleotide identity and encode a protein, suggesting a common origin and role in plasmid evolution.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

A 25-year long-term implementation review of an early intervention for psychosis programme in Hong Kong (part 1): planning and initial development.

East Asian archives of psychiatry : official journal of the Hong Kong College of Psychiatrists = Dong Ya jing shen ke xue zhi : Xianggang jing shen ke yi xue yuan qi kan·2026
Same author

A 25-year long-term implementation review of an early intervention for psychosis programme in Hong Kong (part 2): upscaling, consolidation, and challenges.

East Asian archives of psychiatry : official journal of the Hong Kong College of Psychiatrists = Dong Ya jing shen ke xue zhi : Xianggang jing shen ke yi xue yuan qi kan·2026
Same author

The need for smoking cessation counselling and nicotine withdrawal therapy for hospitalised patients: A smoking point prevalence study at Groote Schuur Hospital, Cape Town, South Africa.

African journal of thoracic and critical care medicine·2024
Same author

Molecular typing of enteroviruses: comparing 5'UTR, VP1 and whole genome sequencing methods.

Pathology·2022
Same author

SARS-CoV-2 Genome Sequencing Methods Differ in Their Abilities To Detect Variants from Low-Viral-Load Samples.

Journal of clinical microbiology·2021
Same author

A self-matched leaky-wave antenna for ultrahigh-field magnetic resonance imaging with low specific absorption rate.

Nature communications·2021

Area of Science:

  • Microbiology
  • Molecular Biology
  • Genetics

Background:

  • Lactococcus lactis is a key bacterium in dairy fermentation.
  • Proteinase activity in L. lactis is crucial for cheese ripening.
  • Plasmids carry genes that confer important traits, such as proteinase production.

Purpose of the Study:

  • To characterize novel DNA segments found on Lactococcus lactis plasmids pWV05 and pSK111.
  • To determine if these DNA segments represent insertion sequence (IS) elements.
  • To investigate the evolutionary relationship between these DNA segments and known IS elements.

Main Methods:

  • DNA sequence analysis of plasmids pWV05 and pSK111.
  • Comparison of nucleotide and amino acid sequences.
  • Identification of open reading frames (ORFs) and conserved domains.

Related Experiment Videos

  • Analysis of flanking regions and genetic context.

    • Main Results:

    • Two highly similar DNA segments, designated ISS1W and ISS1N, were identified on plasmids pWV05 and pSK111, respectively.
    • These segments exhibit 77% nucleotide identity and contain an ORF encoding a 226-amino acid protein.
    • Both segments are located downstream of the prtM gene but have different orientations.
    • ISS1W on pWV05 is partially flanked by another IS element, suggesting plasmid deletion events.

    Conclusions:

    • The characterized DNA segments are proposed to be novel insertion sequence (IS) elements, named ISS1W and ISS1N.
    • The high similarity suggests a common origin or horizontal transfer of these IS elements.
    • The genetic context of ISS1W indicates that plasmid pWV05 may have evolved through deletion of IS elements from a precursor plasmid.