Related Experiment Video
Updated: Jun 1, 2026

07:04
Confocal Time Lapse Imaging as an Efficient Method for the Cytocompatibility Evaluation of Dental Composites
Published on: November 9, 2014
Non-invasive quantification of resin-dentin interfacial gaps using optical coherence tomography: validation against
Turki A Bakhsh1, Alireza Sadr, Yasushi Shimada
1Cariology and Operative Dentistry, Graduate School of Medical and Dental Sciences, Tokyo Medical and Dental University, 1-5-45, Yushima, Bunkyo-ku, Tokyo 113-8549, Japan.
Summary
Swept source optical coherence tomography (SS-OCT) non-invasively detects micrometer gaps at the tooth-restoration interface. This advanced imaging technology accurately quantifies defects, aiding in monitoring composite restorations.
Area of Science:
- Biomaterials Science
- Dental Materials
- Optical Imaging
Background:
- Gap formation at the tooth-restoration interface compromises restoration longevity and can lead to treatment failure.
- Non-destructive assessment methods are crucial for evaluating and monitoring these critical interfacial defects.
Purpose of the Study:
- To evaluate the efficacy of swept source optical coherence tomography (SS-OCT) as a non-invasive technique for assessing the tooth-restoration interface.
- To compare SS-OCT findings with confocal laser scanning microscopy (CLSM) for validation.
Main Methods:
- Class-I cavities in human premolars were restored with an all-in-one adhesive and three composite types (Majesty Posterior, AP-X, Majesty LV).
- Serial cross-sectional images were acquired using SS-OCT (1319 nm), followed by specimen trimming, polishing, and CLSM observation.
- Image analysis software identified signal intensity peaks at the resin-dentin interface, correlating with gap formation.
Main Results:
- Increased SS-OCT signal intensity correlated strongly with interfacial gaps identified by CLSM.
- Interfacial gaps ranged from 26 μm to 1.9 mm in length.
- The universal composite AP-X exhibited the smallest interfacial gaps among the tested materials.
Conclusions:
- SS-OCT is a viable non-invasive technology for detecting and quantifying micrometer-sized gaps at the base of composite restorations.
- SS-OCT holds potential as a valuable monitoring tool for composite restorations in both laboratory research and clinical settings.

