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Receptors for monoamines on cultured cells
1Department of Pharmacology, University of Pennsylvania School of Medicine, Philadelphia 19104-6084.
American Journal of Hypertension
|June 1, 1990
Summary
New pituitary tumor cell lines, SUP1 and P11, provide models for studying dopamine and serotonin receptors. These cell lines express specific receptors, enabling research into monoamine receptor function and expression.
Area of Science:
- Neuroscience
- Molecular Biology
- Endocrinology
Background:
- Clonal cell lines are valuable for studying receptor systems.
- Pituitary tumor 7315a is a source of novel cell lines.
- Monoamine receptors, including dopamine and serotonin receptors, play critical roles in physiological processes.
Purpose of the Study:
- To isolate and characterize clonal cell lines expressing dopamine and serotonin receptors.
- To establish model systems for investigating monoamine receptor function.
- To explore how receptor expression and function can be manipulated.
Main Methods:
- Isolation of clonal cell lines from pituitary tumor 7315a.
- Detection of mRNA for dopamine D-2 receptor in SUP1 cells.
- Assessment of receptor coupling to cellular signaling pathways (cAMP inhibition, phosphoinositide hydrolysis).
Main Results:
- The SUP1 cell line expresses dopamine D-2 receptors, inhibiting cAMP production and prolactin secretion.
- The P11 cell line expresses 5-HT2 receptors, stimulating phosphoinositide hydrolysis.
- These cell lines demonstrate functional expression of specific monoamine receptors.
Conclusions:
- SUP1 and P11 cell lines are suitable models for studying dopamine and serotonin receptors.
- These cell lines will facilitate research into the regulation of monoamine receptor expression and function.
- The characterized cell lines offer a platform for future pharmacological and molecular investigations.