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Mouse Round Spermatid Injection

Mouse Round Spermatid Injection

Published on: January 26, 2024

Methodology matters: IVF versus ICSI and embryonic gene expression.

Phillip J Bridges¹, Myoungkun Jeoung, Heyoung Kim

¹Division of Clinical and Reproductive Sciences, University of Kentucky, Lexington, KY 40536, USA.

Reproductive Biomedicine Online

|June 14, 2011

Summary

Intracytoplasmic sperm injection (ICSI) and ICSI with artificial oocyte activation (ICSI-A) alter gene expression in blastocysts compared to in vitro fertilization (IVF). These genetic changes may explain adverse outcomes associated with assisted reproduction treatments.

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Using Mouse Oocytes to Assess Human Gene Function During Meiosis I

Using Mouse Oocytes to Assess Human Gene Function During Meiosis I

Published on: April 10, 2018

Area of Science:

Reproductive biology
Developmental biology
Genomics

Background:

Assisted reproduction treatments (ART), particularly intracytoplasmic sperm injection (ICSI), are associated with adverse outcomes.
The underlying genetic causes of these adverse effects remain largely unknown.

Purpose of the Study:

To investigate gene expression differences in blastocysts created via ICSI and ICSI with artificial oocyte activation (ICSI-A) compared to conventional in vitro fertilization (IVF).
To provide genetic insights that could aid in diagnosing or treating ART-related adverse effects and refine ART techniques.

Main Methods:

Murine blastocysts were generated using ICSI, ICSI-A, and IVF.
Microarray analysis was employed to assess gene expression profiles.
Differential gene expression and affected biological pathways were identified.

Main Results:

Significant differences in gene expression were observed: 197 genes between ICSI and IVF blastocysts (P < 0.01).
132 genes differed between ICSI-A and IVF blastocysts (P < 0.01).
65 genes differed between ICSI-A and ICSI blastocysts (P < 0.01).
Procedural-induced genetic changes in specific pathways showed correlation with known adverse consequences.

Conclusions:

ICSI and ICSI-A significantly alter blastocyst gene expression compared to IVF.
These genetic alterations may underlie adverse outcomes observed in ART.
Further research into procedure-specific genetic dysfunction is warranted.