Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Stem Cell Niche01:26

Stem Cell Niche

The stem cell niche is the dynamic microenvironment where stem cells reside. Inside these niches, the cells may remain undifferentiated, undergo high self-renewal, or become lineage-specific progenitors. Stem cells coexist with other niche cells, such as stromal cells. They also interact closely with the ECM. Cell-cell and cell-matrix communication occur via adhesion molecules or soluble factors that signal the stem cells and determine their fate. Stromal cells also provide survival signals to...
Multipotency and Niche of Bulge Stem Cell01:06

Multipotency and Niche of Bulge Stem Cell

A hair follicle or HF is a small part of the skin that produces the hair shaft. Paul Gerson Unna was the first to observe a bulge in the human hair follicle's outer root sheath (ORS). The bulge is present between the sebaceous gland and the arrector pili muscle and is the niche for hair follicle stem cells (HFSCs). The bulge is also a niche for melanocyte stem cells, and their loss results in graying of hair. The HFSCs express Sox9 and Lhx2, which help them maintain stemness and prevent...
Maintenance of the ES Cell State01:14

Maintenance of the ES Cell State

The cells of the blastocyst inner cell mass only remain pluripotent for a short time. This state of pluripotency and self-renewal can be maintained in embryonic stem (ES) cell culture by adding specific chemicals or growth factors to ensure the cells can continue dividing and later differentiate into different cell types. In some cases, the cells are grown on a feeder layer of differentiated cells, which provides the growth factors and extracellular matrix components necessary for stem cell...
Zygotic Development And Stem Cell Formation01:10

Zygotic Development And Stem Cell Formation

The development of all multicellular organisms starts with the fusion of haploid cells called sperm and egg to form a diploid zygote. A zygote is a totipotent cell that can develop into a complete organism. The zygote undergoes cell division or cleavage to form an 8-cell mass. Until this stage, the cells are spherical, loosely attached, and remain totipotent. Totipotent cells are capable of developing both the embryonic and the extraembryonic tissues. However, as they continue to divide, they...
Embryonic Stem Cells00:58

Embryonic Stem Cells

Embryonic stem (ES) cells are undifferentiated pluripotent cells, meaning they can produce any cell type in the body. This gives them tremendous potential in science and medicine since they can generate specific cell types for use in research or to replace body cells lost due to damage or disease.
Embryonic Stem Cells00:57

Embryonic Stem Cells

Embryonic stem (ES) cells were first discovered in mice in 1981 by Martin Evans. In 1998, James Thomson identified a method to isolate embryonic stem cells from humans. Human embryonic stem cells (hESCs) are obtained from 3-5 day old embryos that remain unused after an in vitro fertilization procedure.
ES cells are grown in a culture medium where they can divide indefinitely, creating ES cell lines. Under certain conditions, ES cells can differentiate, either spontaneously into a variety of...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Plasma Dilution After Myocardial Ischemia-Reperfusion Injury Promotes Cardiac Repair, Heart Performance, and Recovery of Motor Function and Endurance in Old Mice.

Aging cell·2026
Same author

Human microphysiological systems of aging recreate the in vivo process expediting evaluation of anti-geronic strategies.

Nature biomedical engineering·2026
Same author

Correction for: Sex-specific longitudinal reversal of aging in old frail mice.

Aging·2025
Same author

Oxytocin treatment reduces cancer cachexia in a pre-clinical model.

Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie·2025
Same author

Sex-specific longitudinal reversal of aging in old frail mice.

Aging·2025
Same author

Misalignment of age clocks.

GeroScience·2025

Related Experiment Video

Updated: Jun 1, 2026

Dissection and Live-Imaging of the Late Embryonic Drosophila Gonad
09:08

Dissection and Live-Imaging of the Late Embryonic Drosophila Gonad

Published on: October 17, 2020

Embryonic anti-aging niche.

Irina M Conboy1, Hanadie Yousef, Michael J Conboy

  • 1Department of Bioengineering, QB3 Institute, UC Berkeley, CA 94720-3220, USA. iconboy@berkeley.edu

Aging
|June 14, 2011
PubMed
Summary
This summary is machine-generated.

Aging impairs stem cell function, hindering tissue repair. This study explores how embryonic stem cells rejuvenate aged tissues, offering potential for reversing age-related decline and improving organ regeneration.

More Related Videos

Collection of Serum- and Feeder-free Mouse Embryonic Stem Cell-conditioned Medium for a Cell-free Approach
09:32

Collection of Serum- and Feeder-free Mouse Embryonic Stem Cell-conditioned Medium for a Cell-free Approach

Published on: January 8, 2017

Single Myofiber Culture Assay for the Assessment of Adult Muscle Stem Cell Functionality Ex Vivo
09:19

Single Myofiber Culture Assay for the Assessment of Adult Muscle Stem Cell Functionality Ex Vivo

Published on: February 15, 2021

Related Experiment Videos

Last Updated: Jun 1, 2026

Dissection and Live-Imaging of the Late Embryonic Drosophila Gonad
09:08

Dissection and Live-Imaging of the Late Embryonic Drosophila Gonad

Published on: October 17, 2020

Collection of Serum- and Feeder-free Mouse Embryonic Stem Cell-conditioned Medium for a Cell-free Approach
09:32

Collection of Serum- and Feeder-free Mouse Embryonic Stem Cell-conditioned Medium for a Cell-free Approach

Published on: January 8, 2017

Single Myofiber Culture Assay for the Assessment of Adult Muscle Stem Cell Functionality Ex Vivo
09:19

Single Myofiber Culture Assay for the Assessment of Adult Muscle Stem Cell Functionality Ex Vivo

Published on: February 15, 2021

Area of Science:

  • Stem cell biology
  • Regenerative medicine
  • Aging research

Background:

  • Functional organ stem cells persist in aged individuals, but tissue repair is insufficient, leading to organismal demise.
  • Aging alters regulatory pathways (Notch, MAPK, TGF-β), causing aged tissues to inhibit their own regeneration.
  • Stem cell responses are extrinsically and age-specifically regulated.

Purpose of the Study:

  • To analyze the regulation of organ stem cells by young versus old tissue microenvironments (niches).
  • To investigate the potential of embryonic stem cells to counteract age-related decline in tissue regeneration.
  • To identify strategies for ameliorating or reversing age-imposed decline in stem cell regenerative capacity.

Main Methods:

  • Literature review on stem cell regulation in aging.
  • Analysis of regulatory pathways (Notch, MAPK, TGF-β) in aged tissues.
  • Experimental evidence examining embryonic stem cell pro-regenerative activity.

Main Results:

  • Aged tissue microenvironments inhibit regeneration by altering regulatory pathways.
  • Embryonic stem cells possess an intrinsic youthful barrier to aging.
  • Embryonic stem cells produce pro-regenerative factors that signal the MAPK pathway, rejuvenating myogenesis.

Conclusions:

  • A balance between cell cycle checkpoint proteins and mitogens is crucial for regeneration without tumor formation.
  • Embryonic stem cells offer a model for understanding intrinsic youthfulness and developing rejuvenation strategies.
  • Understanding age-related decline in stem cell regeneration could lead to novel therapies for organ maintenance and reversal of aging.